Aim. The index of creatinine in plasma (serum) of blood, due to simplicity and low cost of its determination, is widely used in routine medical practice to determine the glomerular filtration rate, an integral indicator of renal excretory function. To determine the content of creatinine, various methods have been processed, including the well-known color reaction of Jaffe. The proposed traditional methods require significant time consumption, lengthy and complex sample preparation, expensive reagents, highly qualified personnel and, most importantly, do not provide the possibility of real-time analysis. Therefore, the development of biosensors for creatinine analysis is of great interest. The aim of this work was to study the influence of immobilization method and transduction mode on the analytical characteristics of the biosensors for creatinine determination. Conclusions. The developed electrochemical enzyme biosensors could be adapted to the technologies of large-scale production. Depending on the purpose and conditions of application, one or another transduction mode can be used and the method of creatinine deiminase immobilization can be chosen. The obtained results demonstrate the possibility of their application in medical diagnostics for the detection of creatinine.