Aim. The purpose of the study is to investigate the changes in cytological parameters by changes in the functional state of chromatin of cells of the follicular environment of oocytes under conditions of oxidative stress in vitro, as well as under the influence of SIRT-1 activator, PARP (poly(ADP-ribose) polymerase) blocker, NOS substrate and iNOS blocker, which has not been studied before. Conclusions. Together, the obtained data provide grounds for asserting that both nitric oxide and Sirtuin-1 are involved in maintaining the functional state of chromatin availability in cells of the follicular environment of oocytes.