Biopolym. Cell. 2024; 40(2):127-135.
Molecular Biomedicine
Doxorubicin affects expression of the ZFP36 and CTTN genes in MCF7 cell line
1, 2Hubiernatorova A. O., 1Kropyvko S. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143
  2. Institute of animal physiology and genetics, CAS
    89, Rumburska str., Libechov, Czech Republic, 27721


Aim. The study aims to investigate the effect of doxorubicin treatment of MCF7 cells on the expression of ZFP36, SH3PXD2A, CTTN, SH3PXD2B, WIPF1 and WASL genes coding for TTP, TKS4, TKS5, N-WASP, WIP and CTTN proteins correspondingly. Methods. Cell culture, RT-qPCR, western-blotting and Alamar Blue assay are used in this study. Results. DXR increases both mRNA and protein levels of TTP, which may limit its use as a prognostic marker; it affects the expression of SH3PXD2A, CTTN and WIPF1, but not CTTN and SH3PXD2B. The observed effect is likely an overall response to DNA damage. Conclusion. Doxorubicin significantly affects the expression of ZFP36 and CTTN genes in MCF7 cells, and further investigations are needed to reveal its effects on the cancer cells physiology.
Keywords: doxorubicin, breast cancer, TTP


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