Biopolym. Cell. 1988; 4(6):303-309.
http://dx.doi.org/10.7124/bc.00023D
Cell Biology
Mouse B lymphocyte activation of proliferation and cyclic nucleotides at early stages of activation
1Komissarenko S. V.
  1. A. V. Palladin Institute of Biochemistry, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

Splenocytes and isolated mouse spleen B lymphocytes were activated in culture by: antibodies to μ-chains of mouse immunoglobulins, Fab and F(ab')2 fragments of such antibodies, LPS from E. coli and interleukin 2(IL-2). Cell activation was monitored by [3H]-incorporation and by flow cytofluorimetry. The latter permitted detecting cell-cycle phases distribution of cells stained by acridine orange. It was shown that anti-μ antibodies were cytotoxic to mouse splenocytes and B lymphocytes. Isolated B cell could not be stimulated by Fab or F(ab')2 anti-μ, fragments but were activated by LPS and by F(ab')2 + IL-2, though F(ab')2 were stimulative to mouse splenocytes. In most cases activated cells passed G1 phase and were arrested at the GrS boundary. LPS or F(ab')2 anti-μ, addition to isolated B cells in culture caused a Ca2+-dependent increase in cGMP but not cAMP levels in these cells. Methylene bisphosphonic acid being administered to mice 24 hours before B cells isolation did not change cAMP or cGMP levels in LPS of F(ab')2 activated B lymphocytes. These data show that during the first minutes of B cell activation by LPS or F(ab')2 anti-μ, fragments there occurs a Ca2+-dependent increase in the cGMP level which is not sufficient for these cells proliferation and progression through the whole cell-cycle. The cells are usually arrested in G1 phase.
Full text: (PDF, in Russian)

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