Biopolym. Cell. 1988; 4(5):275-277.
http://dx.doi.org/10.7124/bc.000239
Short Communications
Restriction analyses of dna of fungi Verticillium dahliae Kleb
1Mukhamedov R. S., 1Kholmuratov E. G., 1Abdukarimov A. A.
  1. Institute of Bioorganic Chemistry, Academy of Sciences of the Uzbek SSR
    Tashkent, USSR

Abstract

The protoplasts of fungi Verticillium dahliae Kle.b were obtained, the nuclear DNA was isolated, the restriction analysis by restrictases BamHI, HindIII, BspRI, Sau3A, Sau961 was made. Under these conditions the high repeated sequences of ribosomal genes were probably revealed. The cross-hybridization of DNA from different cotton species and cultivars with DNA of the pathogene fungus revealed a correlation between a degree of damage by Verticillium dahliae and a degree of hybridization of homologous sequences.
Full text: (PDF, in Russian)

References

[1] Krasheninnikov IA, Konoshenko GI, Kulaev IS. On phosphorous compounds in preparations of cell walls from Neurospora crassa mycelia. Dokl Akad Nauk SSSR. 1967;177(3):711-3.
[2] Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977;113(1):237-51.
[3] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning - a laboratory manual. New York, Cold Spring Harbor, 1982; 545 p.
[4] Tarantul VZ, Gol'tsov VA, Kuznetsova ED. Structural organization of the eukaryotic genome. Itogi nauki i tekhniki. Moscow, VINITI, 1982; 22. (Molekulyar. biologiya; Vol. 19).
[5] Krumlauf R, Marzluf GA. Characterization of the sequence complexity and organization of the Neurospora crassa genome. Biochemistry. 1979;18(17):3705-13.
[6] Timberlake WE. Low repetitive DNA content in Aspergillus nidulans. Science. 1978;202(4371):973-5.
[7] Lauer GD, Roberts TM, Klotz LC. Determination of the nuclear DNA content of Saccharomyces cerevisiae and implications for the organization of DNA in yeast chromosomes. J Mol Biol. 1977;114(4):507-26.
[8] Aripdzhanov ShA, Tursunov Kh.The structure of DNA and the stability of cotton to verticillium wilt. Structure and function of nucleic acids and protein biosynthesis in plants: Proc. of reports. II All-Union symp. Pushchino, 1977; 5.