Biopolym. Cell. 1988; 4(3):163-167.
Orientational effects caused in the pUC plasmid by E. coli rplJL-rpoBC-operon fragments
- Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
A number of recombinant pUC plasmids containing E. coli rplJL-rpoBC-operon fragments were constructed. A rplJ gene and PL10 promoter containing 1568 bp EcoRI fragment and rplJ gene structural part containing BspRI-A-fragment were found to be oriented in pUC plasmids unidirectionally, in such a way that genes rplJ of the inserted fragment and lacZ' of the vector were transcribed in the opposite direction.
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