Biopolym. Cell. 1988; 4(3):157-162.
http://dx.doi.org/10.7124/bc.000223
Gene-Engineering Biotechnology
The absence of Ty elements and 2 μm DNA in natural Saccharomyces
1Bulat S. A.
  1. B. P. Konstantinov Institute of Nuclear Physics, Academy of Sciences of the USSR
    Gatchina, Leningrad distr., USSR

Abstract

We have analyzed the spreading of the well-studied repetitive elements of S. cerevislae genome (Ty elements and 2 μm DNA) in the related natural species of S. terrestris. None of the homologous DNA sequences were found in different isolates of S. terrestris. The result is discussed in terms of hypothesis about the eukaryotic repetitive elements role in the species molecular determination.
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References

[1] Williamson VM. Transposable elements in yeast. Int Rev Cytol. 1983;83:1-25.
[2] Roeder G. S., Fink G. R. Transposable elements in yeast. Mobile genet, elements. Ed. J. A. Shapiro. New York: Acad, press, 1983:299-328.
[3] Fink GR, Boeke JD, Garfinkel DJ. The mechanism and consequences of retrotransposons. Trends Genet. 1986; 2, N 5:118-123.
[4] Broach J. R. The yeast piasmid 2 M circle. Mol. biol. of the yeast Saccharomyces: life cycle and inheritance. New York: Cold Spring Harbor Lab., 1981:445-470.
[5] Gunge N. Yeast DNA plasmids. Annu Rev Microbiol. 1983;37:253-76.
[6] Araki H, Jearnpipatkul A, Tatsumi H, Sakurai T, Ushio K, Muta T, Oshima Y. Molecular and functional organization of yeast plasmid pSR1. J Mol Biol. 1985;182(2):191-203.
[7] Utatsu I, Utsunomiya A, Toh-e A. Functions encoded by the yeast plasmid pSB3 isolated from Zygosaccharomyces rouxii IFO 1730 (formerly Saccharomyces bisporus var. mellis). J Gen Microbiol. 1986;132(5):1359-65.
[8] Falcone C, Saliola M, Chen XJ, Frontali L, Fukuhara H. Analysis of a 1.6-micron circular plasmid from the yeast Kluyveromyces drosophilarum: structure and molecular dimorphism. Plasmid. 1986;15(3):248-52.
[9] Storms RK, McNeil JB, Khandekar PS, An G, Parker J, Friesen JD. Chimeric plasmids for cloning of deoxyribonucleic acid sequences in Saccharomyces cerevisiae. J Bacteriol. 1979;140(1):73-82.
[10] Bulat SA, Mezhevaia EV, Stepanova VP, IarovoД­ BF, Zakharov IA. Genetic study of plasmid integration into yeast chromosomes. V. Mapping of integration sites for the plasmid pYF91. Genetika. 1987;23(8):1407-13.
[11] Inge-Vechtomov SG. Yeast. Obshch. Probl. Biologiyi. Moscow, VINITI, 1982; Vol. 1:148-92.
[12] Naumov GI. Biological species Saccharomyces terrestris. Dokl Akad Nauk SSSR. 1979; 249(5):1228-30.
[13] Golubev VI, Blagodatskaya VM, Manukyan AR, Liss OL. Yeast flora of peat. Izv Akad Nauk SSSR Biol. 1981; (2): 181-187.
[14] Sherman F, Fink GR, Lawrence CW. Isolation of yeast nuclear and mitochondrial DNA. Meth. in yeast genet. New York: Cold Spring Harbor Lab., 1984:77-80.
[15] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning - a laboratory manual. New York, Cold Spring Harbor, 1982; 545 p.
[16] Yanisch-Perron C, Vieira J, Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103-19.
[17] Holmes DS, Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981;114(1):193-7.
[18] Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975;98(3):503-17.
[19] Madaule P, Axel R. A novel ras-related gene family. Cell. 1985;41(1):31-40.
[20] Singh N, Kucherlapati R. Detection of specific DNA sequences into somatic cell hybrids and in cells infected with the viral DNA. Methods of somatic cells genetic. Moscow, Mir, 1985; Vol. 2:520-34.
[21] Naumov GI, Nikonenko TA. Divergence genomes of cultivated and wild yeast Saccharomyces sensu stricto: four kinds of double. Dokl Akad Nauk SSSR. 1987; 294(2):476-479.
[22] Naumov GI. Comparative genetics of yeasts. XXIII. Unusual inheritance of toxin formation in Saccharomyces paradoxus batschinskaia. Genetika. 1985;21(11):1794-8.
[23] Naumov GI. Genetic differentiation and ecology of the yeast Saccharomyces paradoxus baischinskaia. Dokl Akad Nauk SSSR. 1986; 291(3):754-7.
[24] Problems of species and genus of fungi. Ed. E Parmasto. Tallinn, Acad. Sci. of the ESSR, 1986; 194 p.
[25] Fulton AM, Mellor J, Dobson MJ, Chester J, Warmington JR, Indge KJ, Oliver SG, de la Paz P, Wilson W, Kingsman AJ, et al. Variants within the yeast Ty sequence family encode a class of structurally conserved proteins. Nucleic Acids Res. 1985;13(11):4097-112.
[26] LaPorte DC. Are the Ty elements of yeast ancient viruses? Trends Biochem. Sci. 1986; 11(7):273-275.
[27] Dowsett AP. Closely related species of Drosophila can contain different libraries of middle repetitive DNA sequences. Chromosoma. 1983;88(2):104-8.
[28] Engels WR. The P family of transposable elements in Drosophila. Annu Rev Genet. 1983;17:315-44.
[29] Bucheton A, Paro R, Sang HM, Pelisson A, Finnegan DJ. The molecular basis of I-R hybrid dysgenesis in Drosophila melanogaster: identification, cloning, and properties of the I factor. Cell. 1984;38(1):153-63.