Biopolym. Cell. 1988; 4(3):151-157.
http://dx.doi.org/10.7124/bc.000222
Gene-Engineering Biotechnology
Obtaining of spheroplasts in nodule bacteria of lucerne, clover, bean and pea
1Bazhenova O. V., 1Potokin I. L., 1Simarov B. V.
  1. All-Union Research Institute of Agricultural Microbiology
    Leningrad, USSR

Abstract

A simple universal procedure has been developed for obtaining stable spheroplasts from the fast-growing species of Rhizobium (R. meliloti, R. phaseoli, R. trifolii, R. leguminosarum). Spheroplasts were prepared by culturing cells in the osmotically stable medium with a decreased content of calcium cations in the presence of penicillin, followed by treatment with lysozyme. The electron-microscopic analysis of the process of spheroplasts formation was performed. The average effectiveness of the cell wall regeneration is 1.4-5.0 %.
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