Biopolym. Cell. 1988; 4(3):133-138.
Structure and Function of Biopolymers
Comparative study of poly(U) and poly(dT) template activity in cell-free protein synthesizing systems from Escherichia coli and wheat germ
1Potapov A. P., 1Soldatkin K. A., 1Soldatkin A. P., 1El'skaya A. V.
- Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
Kiev, USSR
Abstract
To study the role of the codon sugar-phosphate backbone in mRNA decoding process we have compared the messenger activity of authentic ribo- and deoxyribopolynucleotides, poly(U) and poly(dT), in bacterial (E. coli) and plant (wheat germ) cell-free protein-synthesizing systems with and without neomycin (aminoglycoside antibiotic). The effect of neomycin significantly depends on the nature of the cell-free system, magnesium ion and antibiotic concentration. The E. coli system is very sensitive to neomycin. In the presence of neomycin the translation of poly(U) is deeply suppressed, while the translation of poly(dT) is greatly stimulated. The eukaryotic system is relatively insensitive to low concentrations of the antibiotic and unable to translate poly(dT). The results obtained indicate that the translation systems are very sensitive to the structure of the messenger sugars and that the system capacity to translate polynucleotide can be transformed only according to the principle «either ribo- or deoxyribopoly-nucleotide messenger. The transforming agent neomycin does not affect the stage of transpeptidation.
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References
[1]
Potapov AP. A stereospecific mechanism for the aminoacyl-tRNA selection at the ribosome. FEBS Lett. 1982;146(1):5-8.
[2]
Potapov AP. Mechanism of the stereospecific stabilization of codon-anticodon complexes in ribosomes during translation. Zh Obshch Biol. 1985;46(1):63-77.
[3]
Morgan A. R., Wells R. D., Khorana H. G. Studies on polynucleotides. 74. Direct translation in vitro of single-stranded DNA-like polymers with repeating nucleotide sequences in the presence of neomycin B. J Mol Biol. 1967; 26(3):477-497.
[4]
McCarthy BJ, Holland JJ. Denatured DNA as a direct template for in vitro protein synthesis. Proc Natl Acad Sci U S A. 1965;54(3):880-6.
[5]
Bretscher MS. Direct translation of bacteriophage fd DNA in the absence of neomycin B. J Mol Biol. 1969;42(3):595-8.
[6]
Marcus A, Efron D, Weeks DP. The wheat embryo cell-free system. Methods Enzymol. 1974;30:749-54.
[7]
Roberts BE, Paterson BM. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973;70(8):2330-4.
[8]
Soldatkin AP, Zheltovskaia NI, Obcharenko GV, El'skaia AV. Ambiguous polyuridylic acid translation by transport RNA from various eukaryotic substances. Ukr Biokhim Zh. 1983;55(6):603-7.
[9]
de Groot N, Hamburger AD, Lapidot Y. Peptidyl-tRNA. XI. The chemical synthesis of phenylalanine-containing oligopeptidyl-tRNA. Biochim Biophys Acta. 1970;213(1):115-23.
[10]
Salas J, Bollum FJ. Biosynthesis polydeoxynucleotides as direct templates for polypeptide synthesis. J Biol Chem. 1968;243(5):1012-5.
[11]
Pestka S. Inhibitors of protein synthesis. Molecular mechanisms of protein biosynthesis . Eds H. Weissbach, S. Pestka. New York et al. : Acad, press, 1977:467-553.
