Biopolym. Cell. 1988; 4(1):48-53.
http://dx.doi.org/10.7124/bc.000212
Gene-Engineering Biotechnology
Transformation of ltk– aprt– cells by DNA assembled in nucleoprotein complexes
1Zakharian R. A., 1Galstian G. G., 1Gevorkian N. R., 1Galstian M. G., 1Amirkhanova L. M.
  1. Institute of Experimental Biology, Academy of Sciences of the Armenian SSR
    Yerevan, USSR

Abstract

The nucleosome reconstitution was carried out in the presence of DNA, histones and anionic DNA-binding proteins of blood plasma, which serve as a stimulator of self-assembly process. The least sizes of DNA fragment protected from the micrococcal nuclease action corresponds to the DNA value from intact core nucleosome particle. Reconstructed complexes are biologically active in transformation of eukaryotic cells (Ltk–aprt–).
Full text: (PDF, in Russian)

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