Biopolym. Cell. 1988; 4(1):28-34.
http://dx.doi.org/10.7124/bc.00020E
Structure and Function of Biopolymers
Positive topoisomerase activity revealed in a new extreme-thermophilic anaerobic Archaebacterium, reducing sulfur
1Slesarev A. I.
  1. Institute of Molecular Genetics, Academy of Sciences of the USSR
    Moscow, USSR

Abstract

Topoisomerase able to introduce positive supercoils into a closed circular DNA has been first found and partially purified from anaerobic archaebacterium. The topoisomerase fraction obtained by chromatography on phosphocellulose contains two polypeptides with molecular weights 108000 and 135000. ATP, ions Na+ and Mg2+ are needed for the enzyme functioning. Desulfurococcus topoisomerase is not inhibited by O2.
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