Biopolym. Cell. 2023; 39(4):283-298.
Molecular and Cell Biotechnologies
Comprehensive study of hormone-independent highly productive strain of Rauvolfia serpentina tissue culture as a source of indole alkaloids
1Kunakh V. A., 1Konvalyuk I. I., 1Mozhylevska L. P., 1, 2Bieda O. A., 1Twardovska M. O., 1Andreev I. O., 1Yarmolyuk S. M.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143
  2. LLC "Scientific and service firm "Otava"
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143

Abstract

Aim. To characterize a new hormone-independent strain K-27M of R. serpentina tissue culture. Methods. Plant tissue culture, biochemical and cytological analysis, statistical methods. Results. The maximum biomass yield was at the day 69 of subculture, the weight of cell biomass increased 15–18 times over a subculture period. The culture exhibited significant cellular and nuclear polymorphism. The size of cells and nuclei changed during the subculture cycle, thus reflecting the growth stages of cultured tissue. The K-27M strain of R. serpentina (2n=22) was a heterogeneous mixoploid cell population. Modal class consisted of the cells with chromosome numbers ranged from 22 to 33 (more than 65% of all mitotic cells). It was suggested that the cell ploidy is associated with the content of accumulated alkaloids. The maximal content of indole alkaloids was observed from 88 to 108 days of subculture. Conclusions. The K-27M strain of R. serpentina cultured tissue maintained on a simple-composition medium is a prospective producer of indole alkaloids. The estimated daily yield of dry biomass that contains 4% of indole alkaloids including 0.79% of ajmaline is 540 mg per 1 L of media, or 21.5 mg of total indole alkaloids, including 4.3 mg of ajmaline.
Keywords: plant tissue culture, Rauvolfia serpentina, indole alkaloids, cell selection, , , ,

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