Biopolym. Cell. 2022; 38(3):195-204.
http://dx.doi.org/10.7124/bc.000A7E
Bioorganic Chemistry
Study in vitro of the anticancer activity of [3-allyl-4-(41-methoxyphenyl)-3H-thiazole-2-ylidene]-(32-trifluoromethylphenyl)amine hydrobromide toward human tumor cells
1Finiuk N. S., 2Drapak I. V., 2Zimenkovsky B. S., 1Stoika R. S.
  1. Institute of Cell Biology, NAS of Ukraine
    14/16, Drahomanov Str., Lviv, Ukraine, 79005
  2. Danylo Halytsky Lviv National Medical University
    69, Pekarska Str., Lviv, Ukraine, 79010

Abstract

Aim. In vitro study and characterization of anticancer activity of heterocyclic derivative — [3-allyl-4-(41-methoxyphenyl)-3H-thiazole-2-ylidene]-(32-trifluoromethylphenyl)amine hydrobromide. Methods. The cell culture; MTT assay. Results. We synthesized [3-allyl-4-(41-methoxyphenyl)-3H-thiazole-2-ylidene]-(32-trifluoromethylphenyl)amine hydrobromide, which possessed the cardioprotective, as well as the hypolipidemic, anti-inflammatory, analgesic, antihypertensive and antioxidant effects. Here, we investigated its growth inhibitory action towards tumor cell lines of various tissue origins: leukemia (HL-60, Jurkat), liver (HepG2), breast (MCF-7), lung (A549), cervical (KB3-1) and glioma (U251, U373, T98G) cells. We found that the leukemia cells were the most sensitive to the action of [3-allyl-4-(41-methoxyphenyl)-3H-thiazole-2-ylidene]-(32-trifluoromethylphenyl)amine hydrobromide with a mean of IC50 values at 7.5–8.9 μg/mL. Conclusions. The anti-proliferative activity of [3-allyl-4-(41-methoxyphenyl)-3H-thiazole-2-ylidene]-(32-trifluoromethylphenyl)amine hydrobromide dropped in the order: leukemia > hepatocarcinoma ~ cervix > lung carcinoma > glioblastoma > breast carcinoma cells. Thus, we revealed in one molecule of ([3-allyl-4-(41-methoxyphenyl)-3H-thiazole-2-ylidene]-(32-trifluoromethylphenyl)amine hydrobromide) a combination of both the cardioprotective and anticancer activities that is of great significance for this agent as a potent anticancer medicine.
Keywords: [3-allyl-4-(41-methoxyphenyl)-3H-thiazole-2-ylidene]-(32-trifluoromethylphenyl)amine hydrobromide, cytotoxicity in vitro, anticancer activity
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