Biopolym. Cell. 2022; 38(3):158-168.
http://dx.doi.org/10.7124/bc.000A7D
Structure and Function of Biopolymers
Generation of ROS under the influence of thiazole derivative and its complexes with PEG-based polymeric nanoparticles
1Ilkiv M. V., 1Shalai Ya. R., 1Manko B. O., 1Ostapiuk Yu. V., 2Mitina N. E., 2Zaichenko A. S., 1Babsky A. M.
  1. Ivan Franko National University of Lviv
    4, Hrushevskoho Str., Lviv, Ukraine, 79005
  2. Lviv Polytechnic National University
    12, S. Bandery Str., Lviv, Ukraine, 79013

Abstract

Aim. To determine the in vitro effect of thiazole derivative and its complex with polyethylene glycol (PEG)-based nanoscale particles on ROS generation in the NK/Ly lymphoma cells and hepatocytes of mice. Methods. The effects of BF-1 (N-(5-benzyl-1,3-thiazol-2-yl)-3,5-dimethyl-1-benzofuran-2-carboxamide), PEG-based polymeric nanoparticles (Th1, Th3, Th5) and their complexes (Th2, Th4, Th6) on ROS production in murine NK/Ly lymphoma cells were studied using fluorescent microscopy. The level of superoxide in both murine hepatocytes and NK/Ly cells was determined with a spectrophotometric assay. Results. BF1, Th2, Th6 and Th5 significantly increased the level of ROS in NK/Ly lymphoma cells [-] by 27.7 %, 28.6 % 22.7 % and 20.1 %, respectively. Meanwhile, Th1, Th3, Th4 did not affect the ROS level. The level of superoxide significantly decreased under the influence of BF1 by 14.7 % and all its complexes with PEG-based polymeric nanoparticles (Th2, Th4, Th6) by 25.5 %, 21.6 % and 13 %, respectively, compared to control. Unlike lymphocytes, in the murine hepatocytes none of the investigated compounds affected the superoxide content. Conclusions. Thus, thiazole derivative BF1 may realize its antitumor effect on cancer cells by promoting generation of additional amount of ROS. BF1 and its complexes with PEG-containing polymeric nanoparticles significantly increase the ROS generation in NK/Ly cells. Meanwhile, all investigated compounds did not change the level of superoxide in murine hepatocytes. It can be evidence of their low toxicity to nontumor cells.
Keywords: thiazole derivative, polyethylene glycol, polymeric nanoparticles, ROS, superoxide radical
Full text: (PDF, in English)

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