Biopolym. Cell. 2021; 37(6):438-446.
http://dx.doi.org/10.7124/bc.000A69
Molecular and Cell Biotechnologies
Zearalenone-selective biomimetic-based sensor system and its validation for real samples’ analysis
1Yarynka D. V., 1Sergeyeva T. A., 2Piletska E. V., 1Stepanenko Ye. Yu., 3Brovko O. O., 2Piletsky S. A., 1El’skaya A. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143
  2. University of Leicester
    University Road, Leicester LE1 7RH, UK
  3. Institute of Macromolecular Chemistry, NAS of Ukraine
    48, Kharkivske Shosse, Kyiv, Ukraine, 02160

Abstract

Aim. The paper presents the calibration and validation of a recent biomimetic-based fluorescent sensor system for the analysis of zearalenone (ZON) contamination in cereals. Methods. ZON-specific biomimetic sensing elements in a form of molecularly imprinted polymer (MIP) membrane were prepared by the in-situ polymerization method using cyclododecyl-2,4-dihydroxybenzoate as a dummy template molecule and ethylene glycol methacrylate phosphate (EGMP) as a functional monomer. The detection is based on the fluorescence of ZON selectively adsorbed on the MIP membrane surface, which is registered by the spectrofluorimeter. Results. A biomimetic-based sensor system was calibrated for zearalenone analysis in cereal food products. The working range of the sensor system as well as its detection limit and the selectivity were examined. The calibrated biomimetic-based sensor system was successfully validated using maize and wheat flour with mean recoveries about 100 %. Conclusions. Calibration and validation studies of the novel ZON-selective biomimetic-based sensor system were successfully performed in cooperation with SE Ukrmetrteststandard. The standardized biomime-tic-based fluorescent sensor system holds great potential to provide reliable prevention instrument against ZON contamination in cereals-based food products and feeding stuff.
Keywords: zearalenone, molecularly imprinted polymer membranes, biomimetic sensor system
Full text: (PDF, in English)

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