Biopolym. Cell. 2021; 37(6):419-427.
http://dx.doi.org/10.7124/bc.000A67
Structure and Function of Biopolymers
Donor variability of the Wharton's jelly-derived MSCs in response to oxidative stress
1, 2Kovalchuk M. V., 2Shuvalova N. S., 1, 2Kordium V. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143
  2. State Institute of Genetic and Regenerative Medicine, NAMS of Ukraine
    67, Vyshhorodska Str., Kyiv, Ukraine, 04114

Abstract

Aim. Since cell survival is crucial for the therapeutic efficacy, it is important to assess the MSC inter-donor variability under oxidative conditions. This study was undertaken to examine the functional responses to the oxidative stress of Wharton jelly-derived MSCs (WJ‑MSCs) from different donors. Methods. MSCs were obtained by the explant method and cultured accor-ding to standard methods. The oxidative stress was induced by hydrogen peroxide (H2O2). Using the MTT assay the treated WJ‑MSCs were analyzed for metabolic activity and survival. Results. Our findings indicated that viability of individual WJ‑MSCs was affected by H2O2 in a biphasic mode at concentrations between 6.25 and 440 µM for most cell samples. At lower H2O2concentrations, 6.25–50 μM, an increase in the viability to 16 % was noted. The maximum stimulating effect was observed at concentrations of 12.5 μM and 25 μM depending on the donor. We found that the preconditioning of WJ‑MSCs with 12.5 and 25 μM H2O2 for 24 h enhanced their survival under toxic H2O2-doses and survival rates varied for different donors. Conclusions. The findings indicated that the WJ‑MSC responses to H2O2-induced oxidative stress varied for different donors. The viability of H2O2-preconditioned WJ‑MSCs changed in a donor-dependent manner in severe environment. Consequently, to produce effective medical cells it is important to consider the donor variability in the process of oxidative MSC conditioning.
Keywords: MSCs, donor variability, oxidative stress, hormesis
Full text: (PDF, in English)

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