Biopolym. Cell. 2021; 37(5):346-356.
http://dx.doi.org/10.7124/bc.000A60
Molecular and Cell Biotechnologies
Validation of aflatoxin B1 MIP membrane-based smartphone sensor system for real sample applications
1Yarynka D. V., 1Sergeyeva T. A., 2Piletska E. V., 3Linnik R. P., 4Antonyuk M. Z., 5Brovko O. O., 2Piletsky S. A., 1El’skaya A. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143
  2. University of Leicester
    University Road, Leicester LE1 7RH, UK
  3. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01601
  4. National University of "Kyiv-Mohyla Academy"
    2, Skovorody Str., Kyiv, Ukraine, 04655
  5. Institute of Macromolecular Chemistry, NAS of Ukraine
    48, Kharkivske Shosse, Kyiv, Ukraine, 02160

Abstract

Aim. To calibrate and validate of innovative MIP membrane-based smartphone sensor system for aflatoxin B1 detection in real cereal samples. Methods. Porous molecularly imprinted polymer (MIP) membranes were synthesized according to the technique of molecular imprinting using the method of in situ polymerization. As a dummy template, ethyl-2-oxocyclopentanecarboxylate was used. Acrylamide was used as a functional monomer according to the results of molecular dynamics. Porous 60 µm-thick MIP membranes were obtained using UV polymerization for 30 min (Sergeyeva et al. 2017). The Fluorescent sensor responses of AFB1 binding to the selective MIP membrane surface were recorded by the spectrofluorimeter as well as smartphone camera and further analyzed by smartphone application Spotxel® Reader (Sicasys Software GmbH, Germany). Results. The calibration plots for both MIP membrane-based fluorescent sensor system and smartphone sensor system were obtained. The possibility of successful application of the proposed sensor systems to analyze AFB1 in real samples was demonstrated. Conclusions. The MIP membrane-based smartphone sensor was calibrated and validated for reliable and robust aflatoxin B1 detection in the real cereal samples. The proposed MIP membrane-based smartphone sensor system provides affordable, sensitive, and equipment-free technique for AFB1 analysis compared to the traditional analytical methods.
Keywords: aflatoxin B1, molecularly imprinted polymer membranes, smartphone-based sensors
Full text: (PDF, in English)

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