Biopolym. Cell. 2021; 37(3):167-176.
http://dx.doi.org/10.7124/bc.000A54
Viruses and Cell
N-stearoylethanolamine - a new inhibitor of the hepatitis C virus reproduction
1Hula N. M., 1Chumak A. A., 1Berdyshev A. G., 1Kosiakova G. V., 1Goridko T. M., 1Meged’ O. F., 1Asmolkova V. S., 2Porva Yu. I., 2Rybalko S. L., 2Dyadun S. T., 2Starosyla D. B., 2Deryabin O. M.
  1. Palladin Institute of Biochemistry, NAS of Ukraine
    9, Leontovycha Str., Kyiv, Ukraine, 01601
  2. Gromashevsky L. V. Institute of Epidemiology and Infection Diseases, NAMS of Ukraine
    5, Amosova Str., Kyiv, Ukraine, 03038

Abstract

Aim. The study of the effect of endogenous cannabimimetic compound – N-stearoylethanolamine (NSE) on the hepatitis C virus (HCV) reproduction. Methods. The model of the surrogate HCV is a bovine diarrhea virus; cell culture model is cells transfected with cDNA of the human HCV and molecular docking has been used. Results. In vitro studies showed that NSE effectively inhibited the reproduction of a surrogate HCV in both MDBK cells and transfected Jurkat cells. Molecular docking suggested that NSE can bind to the active centers of both NS3 serine protease and HCV NS5B-polymerase and has an inhibitory effect on their activity. Conclusions. The obtained data confirm that using NSE is promising for the development of antiviral drug to suppress the HCV activity.
Keywords: N-stearoylethanolamine, antiviral activity, hepatitis C virus, molecular docking
Full text: (PDF, in English)

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