Biopolym. Cell. 2018; 34(5):367-373.
Molecular and Cell Biotechnologies
Amperometric glucose biosensor with the IrNPs/Ludox – modified enzyme matrix
1Shkotova L. V., 3Woloshina I. M., 2Kovalchuk V. V., 1Zhybak M. T., 1, 2Dzyadevych S. V.
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143 - Institute of High Technologies,
Taras Shevchenko National University of Kyiv
2, korp.5, Pr. Akademika Hlushkova, Kyiv, Ukraine, 03022 - Kyiv National University of Technologies and Design
2, Nemirovich-Danchenko Str., Kyiv, Ukraine, 01011
Abstract
Aim. To develop an amperometric biosensor based on glucose oxidase (1.1.3.4) from Aspergillus niger immobilized in the IrNPs/Ludox/GOx matrix for glucose detection. Methods. To achieve a highly selective and sensitive glucose detection, the enzymatic membrane was functionalized with Ir nanoparticles (IrNPs) and silica composite Ludox. The enzymatic selective layer was formed on the surface of a platinum disk electrode using immobilization in glutaraldehyde vapor. Results. The voltamperometric characteristics of the transducers with modified IrNPs/Ludox/GOx matrix were studied. Enzyme immobilization on the surface of amperometric transducers was optimized to perform sample analysis. Modified transducers improved biosensor sensitivity. The analytical characteristics of amperometric transducer were determined: detection limit is 0.1 µM (s/n = 3), linear working range is 0.05–3.2 mM, sensitivity is 106 mA×M–1×cm–2. Conclusions. Application of the matrix modified with Ir nanoparticles and silica composite Ludox was investigated for the amperometric glucose biosensor as the most studied model of biosensors. A significant increase in the biosensor sensitivity was obtained using the new approach of glucose oxidase immobilization; therefore application of the matrix modified with mesoporous silica composite and nanometals opens new possibilities to obtain a bioselective membrane of high sensitivity and stability at the development of new electrochemical biosensors.
Keywords: amperometric biosensor, Ir nanoparticles, silica, glucose oxidase
Full text: (PDF, in English)
References
[2]
Kong T, Chen Y, Ye Y, Zhang K, Wang Z, Wang X. An amperometric glucose biosensor based on the immobilization of glucose oxidase on the ZnO nanotubes. Sens Actuators B Chem. 2009;138(1):344−50.
[3]
Park S, Boo H, Chung TD. Electrochemical non-enzymatic glucose sensors. Anal Chim Acta. 2006;556(1):46-57.
[4]
Bai L, Yuan R, Chai Y, Zhuo Y, Yuan Y, Wang Y. Simultaneous electrochemical detection of multiple analytes based on dual signal amplification of single-walled carbon nanotubes and multi-labeled graphene sheets. Biomaterials. 2012;33(4):1090-6.
[5]
Zhai D, Liu B, Shi Y, Pan L, Wang Y, Li W, Zhang R, Yu G. Highly sensitive glucose sensor based on pt nanoparticle/polyaniline hydrogel heterostructures. ACS Nano. 2013;7(4):3540-6.
[6]
Yang Z, Zong X, Ye Z, Zhao B, Wang Q, Wang P. The application of complex multiple forklike ZnO nanostructures to rapid and ultrahigh sensitive hydrogen peroxide biosensors. Biomaterials. 2010;31(29):7534-41.
[7]
El-Refaei SM, Saleh MM, Awad MI. Enhanced glucosee at a binary catalyst of manganese and nickel oxides modified glassy carbon electrode. J Power Sources. 2013;223:125−8.
[8]
Dai Z, Liu S, Ju H, Chen H. Direct electron transfer and enzymatic activity of hemoglobin in a hexagonal mesoporous silica matrix. Biosens Bioelectron. 2004;19(8):861-7.
[9]
Bharathi S, Nogami M. A glucose biosensor based on electrodeposited biocomposites of gold nanoparticles and glucose oxidase enzyme. Analyst. 2001;126(11):1919-22.
[10]
Rao Chepuri RK, Trivedi DC. Chemical and electrochemical depositions of platinum group metals and their applications. Coord Chem Rev. 2005;249(5-6):613–31.
[11]
Shkotova LV, Slast'ia EA, Zhyliakova TA, Soldatkin OP, Schuhmann W, Dziadevych SV. [Amperometric biosensor for ethanol analysis in wines and grape must during wine fermentation]. Ukr Biokhim Zh (1999). 2005;77(1):96-103.
[12]
Manca P, Scanu R, Zucca A, Sanna G, Spano N, Pilo M. Electropolymerization of a Ru(II)–terpyridine complex ethynyl–terthiophene functionalized originating different metallopolymers. Polymer. 2013;54(14):3504–9.
