Biopolym. Cell. 2018; 34(3):218-228.
Bioorganic Chemistry
Anti-HIV activity of ellagitannins from alder tree fruits
1Trokhymchuk T. Yu., 2Shalamay A. S., 3Zavelevich M. P., 4Palchykovska L. G., 4Vasylchenko O. V., 5Rybalko S. L., 5Starosyla D. B., 5Diadiun S. T.
  1. Joint-Stock Company "Diaproph-Med"
    35, Svetlitsky str., Kyiv, Ukraine, 04123
  2. Borschagovsky CPP, Kyiv, Ukraine
    17, Myru Ыtr. 17, Kyiv, Ukraine, 03134
  3. R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
    45, Vasilkivska Str., Kyiv, Ukraine, 03022
  4. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143
  5. Gromashevsky L. V. Institute of Epidemiology and Infection Diseases, NAMS of Ukraine
    5, Amosova Str., Kyiv, Ukraine, 03038


Aim. To assess the inhibitory activity of ellagitannin complex of the purified extract from the fruits of white alder and black alder in non-eukaryotic polymerase systems and to analyze the effects of these substances on the reverse transcriptase of murine leukemia virus and HIV in vitro. Methods. Chronically infected MT4/BIII culture of human leukemic cells was used as the HIV source. Anti-HIV activity of the ellagitannin-containing extract was estimated by the reduction of HIV infectious titer in the MT-4 cells. The inhibitory effects of the extract on retroviral reverse transcriptase, T7 RNA-polymerase and Taq polymerase were also studied in cell-free systems. Results. The ellagitannin extract inhibited activities of the reverse transcriptase of murine leukemia retrovirus, T7 RNA-polymerase, and Taq polymerase. It also reduced the HIV infectious titer in MT-4 cells with IC 50 of 1.5 μg/ml and selectivity index of 87. Conclusions. The ellagitannin complex possesses a significant anti-HIV activity. Its in vitro antiviral effect is achieved with a relatively low toxicity
Keywords: ellagitannins, HIV, anti-HIV activity, reverse transcriptase, T7 RNA-polymerase, Taq-polymerase


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