Biopolym. Cell. 2017; 33(6):434-441.
http://dx.doi.org/10.7124/bc.00096A
Genomics, Transcriptomics and Proteomics
Cardiospecific knockout of αE-catenin leads to violation of the neonatal cardiomyocytes maturation via β-catenin and Yap signaling
1Balatskyi V. V., 1Ruban T. P., 1Macewicz L. L., 1Piven O. O.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Aim. To study the αE-catenin gene function in neonatal cardiomyocytes proliferation and maturation. Methods. Conditional knockout approach, histological (H&E and acridine orange staining) and molecular genetics (qPCR) methods were used. Result. The hetero- and homozygous embryonic cardiospecific knockout of αE-catenin is associated with an increased level of neonatal cardiomyocytes proliferation and a decreased binucleated cells frequency. Knockout of αE-catenin leads to up-regulation of the β-catenin- and Yap-target genes expression (Axin2, c-Myc, Tcf-4, CyclinD1, Ctgf and Tnfrsf1b). Conclusion. αE-catenin is involved in the regulation of proliferation and maturation of neonatal cardiomyocytes via modulation of the activity of β-catenin- and Yap-dependent transcription.
Keywords: heart maturation, αE-catenin, Wnt signaling, HIPPO signaling, cardiomyocyte
Full text: (PDF, in English)

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