Biopolym. Cell. 2017; 33(6):403-414.
Structure and Function of Biopolymers
The dynamics of actin filament polymerization in activated leukocytes under experimental diabetes mellitus against the background of agmatine administration
1Brodyak I. V., 1Bila I. I., 1Sybirna N. O.
  1. Ivan Franko National University of Lviv
    4, Hrushevskoho Str., Lviv, Ukraine, 79005


Aim. To research the impact of agmatine on the redistribution of actin fractions, which are repre-sented by cytoskeletal actin filaments, short actin filaments of the plasma membrane skeleton and actin monomers (G-actin) in rat leukocytes under experimental diabetes mellitus (EDM). Methods. Leukocytes were lyzed in Triton X-100 and subjected to centrifugation to obtain cytoskeletal actin filaments, short actin filaments and actin monomers, which were separated in SDS-PAAG, followed by the immunoblot analysis of anti-actin antibodies. Results. Under EDM, an intensifi-cation of the process of short actin filament depolymerization and an increase in the G-actin con-tent were observed in leukocytes activated by sialospecific wheat germ lectin (WGA). Against a background of agmatine administration, the response dynamics to the WGA-stimulating effect was characterized by an increase in actin polymerization in the fraction of cytoskeletal filaments already after 0.5 min exposure to lectin, an exceptionally rapid depolymerization process after 1 min of the lectin treatment, and return to the initial indices after 3 min exposure to lectin. Conclusions. In leukocytes of animals with EDM against a background of agmatine administration, the transduction of WGA-induced signal through sialoglycoconjugates causes the actin redistribution. It indicates that this polyamine helps to restore and maintain a functional response of leukocytes to the activation signals.
Keywords: actin, leukocytes, agmatine, experimental diabetes mellitus


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