Biopolym. Cell. 2017; 33(1):58-63.
http://dx.doi.org/10.7124/bc.000945
Viruses and Cell
Restriction analysis and differentiation of Ukrainian strains of infectious bursal disease virus
1, 2Pastyria A. S., 2Sobko I. O., 1Polischuk V. P.
  1. Educational and Scientific Center "Institute of Biology",
    Taras Shevchenko National University of Kyiv
    64/13, Volodymyrska Str., Kyiv, Ukraine, 01601
  2. Center of Veterinary Diagnostics
    25-A, Ushinskogo stк., Kyiv, Ukraine

Abstract

The infectious bursal disease virus (IBDV) had a great influence on poultry industry since emergence of very virulent strains in 1980s. The virus leads to immunosuppression and high mortality. There are many different vaccines against IBDV, so characterization of field strains is needed. Aim. To characterize and differentiate vaccine and field virus strains using restriction analysis. Methods. RNA was extracted using sorption method. After reverse transcription, DNA was amplified using PCR with specific primers to VP2 gene. The obtained amplicons were digested with a set of restriction enzymes (MvaI, MboI, SspI, BspMI, SacI, BstEII). Results. The restriction profile of eight vaccine strains was described and 120 bursa samples from infected birds were analyzed. Most of the analyzed virus isolates had restriction profiles similar to vaccine strains. Vaccine strains belonging to the same group in terms of attenuation had a similar restriction profile. Four field isolates have been classified as vvIBDV. They were differentiated from vaccine strains by the presence of SspI and the absence of BstEII sites. Restriction analysis is appropriate to differentiate vaccine and field IBDV isolates. Conclusion. Our approach can be used to monitor vvIBDV in poultry farms.
Keywords: IBDV, field strains, vvIBDV, restriction analysis
Full text: (PDF, in English)

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