Biopolym. Cell. 2016; 32(5):367-376.
http://dx.doi.org/10.7124/bc.000933
Molecular and Cell Biotechnologies
Effect of Ceftriaxone and Timentin antibiotics on morphogensis in the in vitro culture of bread wheat Triticum aestivum L.
1, 2Bannikova M. A., 1Gorbatyuk I. R., 1, 2Hnatiuk I. S., 3Malysheva-Otto L. V., 1Duplij V. P.
  1. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. National Technical University of Ukraine "Igor Sikorsky Kyiv Polytechnic Institute"
    37, Pobedy Av., Kyiv, Ukraine, 03056
  3. IT-Breeding GmbH
    Am Schwabeplan 1b, Gatersleben, Germany, 06466

Abstract

Aim. To study the influence of antibiotics Ceftriaxone and Timentin on the morphogenic calli and roots formation, shoots regeneration in two bread wheat cultivars Triticum aestivum L., to study effect of Ceftriaxone on the growth dynamics of plants. Methods. In vitro plant tissue culture, analysis of Variance, correlation and regression analysis. Results. The influence of β-lactam antibiotics Ceftriaxone and Timentin on morphogenetic processes (morphogenic calli formation, shoots regeneration and rhizogenesis), was studied in apical 18-day-old wheat calli. Two wheat genotypes of different growth habits, winter and winter-spring, were used. Conclusions. Timentin and Ceftriaxone stimulate morphogenic calli formation in bread wheat apical calli. As compared to Timentin, Ceftriaxone has strong concentration-dependent and genotype-dependent influence on shoots regeneration. The roots formation depended primarily on the wheat genotype, independently from an antibiotic applied. The presence of Ceftriaxone in culture medium stimulates rooting and growth of regenerating plants as well as their biomass increment.
Keywords: Timentin, Ceftriaxone, morphogenic callus, shoots regeneration, root formation, Triticum aestivum L.
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Supplementary data

References

[1] Yarizade A, Aram F, Niazi A, Ghasemi Y. Evaluation of effect of β-lactam antibiotics on suppression of different strains of Agrobacterium tumefaciens and on wheat mature embryo culture. Iran J Pharm Sci. 2012; 8(4):267–76.
[2] Fallah-Ziarani M, Haddad R, Garoosi Gh, Jalali M. Agrobacterium-mediated transformation of cotyledonary leaf of lettuce (Lactuca sativa L.) by the GCHI gene. Iran J Pharm Sci. 2013; 2(2):47–55.
[3] Tang H, Ren Z, Krczal G. An evaluation of antibiotics for the elimination of Agrobacterium tumefaciens from walnut of somatic embryos and for the effects on the proliferation of somatic embryos and regeneration of transgenic plants. Plant Cell Rep. 2000;19(9):881–7
[4] Zhao X, Zhan L-P, Zou X-Z. Improvement of cold tolerance of the half-high bush Northland blueberry by transformation with the LEA gene from Tamarix androssowii. Plant Growth Regul. 2011; 63(1):13–22.
[5] Duan HY, Ding XS, Song JY, He YL, Zhou YQ. Plant regeneration and Agrobacterium-mediated transformation of Achyranthes bidentata using cotton EREBP gene. Braz Arch Biol Technol. 2013; 56(3):349–56.
[6] Priya A, Pandian SK, Manikandan R. The effect of different antibiotics on the elimination of Agrobacterium and high frequency Agrobacterium-mediated transformation of indica rice (Oryza sativa L.). Czech J Genet Plant Breed. 2012; 48(3):120–30
[7] Palla KJ, Pijut PM. Agrobacterium-mediated genetic transformation of Fraxinus americana hypocotyls. Plant Cell Tiss Organ Cult. 2015; 120(2):631–41.
[8] Mamidala P, Nanna R. Influence of antibiotics on regeneration efficiency in tomato. Plant Omics J. 2009; 2(4):135–40.
[9] Han S-N, Oh P-R, Kim H-S,Heo H-Y, Jun Cheol Moon, Lee S-K, Kim K-H, Seo Y-W, Lee B-M. Effects of antibiotics on suppression of Agrobacterium tumefaciens and plant regeneration from wheat embryo. J Crop Sci Biotech. 2007; 10(2):92–8.
[10] Nauerby B, Billing K, Wyndaele R. Influence of the antibiotic timentin on plant regeneration compared to carbenicillin and cefotaxime in concentrations suitable for elimination of Agrobacterium tumefaciens. Plant Sci. 1997; 123(1-2):169–77.
[11] Costa MGC, Nogueira FTS, Figueira ML, Otoni WC, Brommonschencel SH, Cecon PR. Influence of the antibiotic Timentin on plant regeneration of tomato (Lycopersicon esculentum Mill.) cultivars. Plant Cell Rep. 2000; 19(3):327-32.
[12] Grzebelus E, Skop L. Effect of β-lactam antibiotics on plant regeneration in carrot protoplast cultures. In Vitro Cell Dev Biol Plant. 2014;50(5):568-75.
[13] Haddadi F, Aziz MA, Abdullah SN, Tan SG, Kamaladini H. An efficient Agrobacterium-mediated transformation of strawberry cv. Camarosa by a dual plasmid system. Molecules. 2015;20(3):3647-66.
[14] Li Z, Liu G, Fang F, Bao M. Adventitious shoot regeneration of Platanus acerifolia Willd. facilitated by Timentin, an antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation. For Stud China. 2007; 9(1):14–8.
[15] Tambarussi EV, Rogalski M, Nogueira FTS, Brondani GE, De Martin VF, Carrer H. Influence of antibiotics on indirect organogenesis of Teak. Ann For Res. 2015; 58(1):177–83.
[16] Gorbatyuk IR, Hnatyuk JS, Bannikov NA Taranenko AM, Morgun BV. Influence of growth regulators on callus regenerative ability wheat varieties Zymoyarka. Fiziol Rast Genet. 2015; 47(6):514–25.
[17] Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962; 15:473–97.
[18] Gamborg OL, Eveleigh DE. Culture methods and detection of glucanases in cultures of wheat and barley. Can J Biochem. 1968; 46(5):417–21.
[19] Sidorov V, Duncan D. Agrobacterium-mediated maize transformation: immature embryos versus callus. Methods Mol Biol. 2009;526:47-58.
[20] Ihaka R, Gentleman R. A language for data analysis and graphics. J Comput Graph Stat. 1996; 5(3):299–314.
[21] Holford P, Newbury HJ. The effects of antibiotics and their breakdown products on the in vitro growth of Antirrhinum majus. Plant Cell Rep. 1992;11(2):93-6.
[22] Ling H-Q, Kriseleit D, Ganal MW. Effect of ticarcillin/potassium clavulanate on callus growth and shoot regeneration in Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum Mill.). Plant Cell Rep. 1998; 17(11):843–7.
[23] Souza Canada ED, Beck E. Embryogenic callus induction on the scutellum and regeneration of plants as basis for genetic transformation of spring wheat (Triticum aestivum L.) cultivars from Argentina. BAG J Basic Appl Genet. 2013; 24(2):55–66.