Biopolym. Cell. 2016; 32(4):262-270.
http://dx.doi.org/10.7124/bc.000928
Molecular and Cell Biotechnologies
Morphological characteristics of mesenchymal stem cells from Wharton jelly, cultivated under physiological oxygen tensions, in various gas mixtures
1Shuvalova N. S., 1, 2Kordium V. A.
  1. State Institute of Genetic and Regenerative Medicine, NAMS of Ukraine
    67, Vyshhorodska Str., Kyiv, Ukraine, 04114
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Cultivation of mesenchymal stem cells (MSC) under physiological oxygen tensions has beneficial effect on their properties. Aim. To examine the effects of mild hypoxic conditions on the morphologic behavior of MSC from human Wharton jelly (WJ-MSC). Methods. WJ-MSC were obtained by an explant method and cultured in gas mixtures containing 3 % O2, 4 % CO2 and 93 % nitrogen or argon. At each passage the cultures were fixed and stained with hematoxylin and eosin. The morphometric analysis of microphotographs was performed, and the nuclear-cytoplasmic ratio (NCR) and the width/length ratio were calculated. Results. The difference in NCR and the width/length ratio was detected between WJ-MSC cultures at various passages and between the cultures at the same passage, maintained in different gas mixtures. The highest level of morphological homogeneity was observed at passage 2. At passage 3, the cells with senescent morphology can be observed. The degree of heterogeneity in populations cultured at 3 % O2 appeared to be lower than under standard conditions. WJ-MSC, cultured in the nitrogen-based gas mixture were the most homogeneous. Conclusions. The changes in WJ-MSC culture morphology were observed at early passages. The level of morphological heterogeneity varied with the cultivation time. The conditions of mild hypoxia had a positive impact on the cultured cells.
Keywords: mesenchymal stem cells, Wharton jelly, morphology, hypoxia, argon
Full text: (PDF, in English)

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