Biopolym. Cell. 2016; 32(3):229-234.
http://dx.doi.org/10.7124/bc.000925
Molecular and Cell Biotechnologies
Development of amperometric biosensor for choline determination
1, 2Kucherenko D. Yu., 3Siediuko D. V., 2Knyzhnykova D. V., 1, 2Soldatkin O. O., 1, 2Soldatkin A. P.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of High Technologies,
    Taras Shevchenko National University of Kyiv
    2, korp.5, Pr. Akademika Hlushkova, Kyiv, Ukraine, 03022
  3. National Aviation University
    1, Komarova av, Kyiv, Ukraine, 03058

Abstract

Aim. Development of an amperometric biosensor for measuring choline concentration in water samples. Methods. A bioselective element of the biosensor was created using choline oxidase which was covalently immobilized by glutaraldehyde crosslinking with bovine serum albumin on the surface of an amperometric platinum disk electrode. Results. The conditions of the bioselective element formation (the enzyme and glutaraldehyde concentrations, time of procedure) were optimized. The biosensor developed was characterized by good response reproducibility over hours of continuous operation. The linear range of substrate determination ranged from 10 µM to 1000 µM, a limit of choline detection – 1–3 µM, the biosensor sensitivity was 25–30 nA/mM. An effect of interfering substances was significantly reduced by the application of an additional semipermeable poly-m-phenylenediamine (PPD) membrane. Conclusions. The developed biosensor is well-suited for choline determination in water samples.
Keywords: biosensor, amperometric transducer, immobilized enzyme, choline oxidase, choline
Full text: (PDF, in English)

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