Biopolym. Cell. 2016; 32(1):3-8.
http://dx.doi.org/10.7124/bc.000906
Reviews
DNA metabarcoding of microbial communities for healthcare
1Zaets I. Ye., 1Podolich O. V., 2Reva O. N., 1Kozyrovska N. O.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Department of Biochemistry, Bioinformatics and Computational Biology Unit, University of Pretoria
    Lynnwood road, Hillcrest, Pretoria, South Africa, 0002

Abstract

High-throughput sequencing allows obtaining DNA barcodes of multiple species of microorganisms from single environmental samples. Next Generation Sequencing (NGS)-based profiling provides new opportunities to evaluate the human health effect of microbial community members affiliated to probiotics. The DNA metabarcoding may serve to a quality control of microbial communities, comprising complex probiotics and other fermented foods. A detailed inventory of complex communities is a pre-requisite of understanding their functionality as whole entities that makes it possible to design more effective bio-products by precise replacement of one community member by others. The present paper illustrates how the NGS-based DNA metabarcoding aims at the profiling of both wild and hybrid multi-microbial communities with the example of kombucha probiotic beverage fermented by yeast-bacterial partners.
Keywords: DNA metabarcoding, microbial communities, healthcare, probiotics
Full text: (PDF, in English)

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