Biopolym. Cell. 2015; 31(5):379-386.
Molecular and Cell Biotechnologies
Development of monoclonal antibody against protein Rictor, a component of the mTORC2 complex
1Malanchuk O. M.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


Aim. Rictor is a component of the protein complex mTORC2 that is activated by growth factors and regulates cell growth, survival and migration. Here, we describe the development of the Rictor specific monoclonal antibody and characterize its suitability for various immunological assays. Methods. Hybridoma technology has been used for the monoclonal antibody production. Immunization was carried out with the recombinant N-terminal fragment of human Rictor expressed in E. coli as a GST-tagged fusion protein. Results. Specific monoclonal antibody (mAb) against Rictor has been developed. Conclusions. The generated mAb specifically recognizes the recombinant and endogenous Rictor and is suitable for Western blotting, immunoprecipitation, and immunofluorescence assays of mammalian cells. This mAb will be a useful tool for the investigations of a physiological role of Rictor as well as the function of the mTORC2 in general.
Keywords: Rictor, mTORC2, hybridoma technique, monoclonal antibody


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