Biopolym. Cell. 2015; 31(3):233-239.
http://dx.doi.org/10.7124/bc.0008E5
Methods
Comparison of proliferative activity of Wharton jelly mesenchymal stem cells in cultures under various gas conditions
1Shuvalova N. S., 1, 2Kordium V. A.
  1. State Institute of Genetic and Regenerative Medicine, NAMS of Ukraine
    67, Vyshhorodska Str., Kyiv, Ukraine, 04114
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Aim. To optimize the cultivation of Wharton jelly-derived mesenchyma stem cells (WJ-MSCs) using physiological oxygen concentrations, and to compare the effect of “hypoxic” gas mixtures, based on nitrogen and argon, on their proliferative activity. Methods. From the first passage, WJ-MSCs were cultivated during five passages in the nitrogen-based gas mixture (3 % oxygen, 4 % carbon dioxide, 93 % nitrogen) and argon-based gas mixture (3 % oxygen, 4 % carbon dioxide, 93 % argon), 7 days before replating. At each passage the final cell number was estimated and the number of population doublings was calculated. Results. The proliferation level of WJ-MSCs, cultured in both gas mixtures with 3 % of O2, was significantly higher compared to that under the regular CO2-incubator conditions. In argon-based mixture, the WJ-MSCs proliferation was higher than in the control but lower than in nitrogen-based mixture. Conclusion. Cultivation of human WJ-MSCs under 3 % O2 had a stimulating effect on the cell proliferation potential. The highest intensity of the cell multiplication was observed in the nitrogen-based mixtures.
Keywords: mesenchymal stem cells, Wharton jelly, hypoxia, physiological oxygen concentration, proliferation
Full text: (PDF, in English)

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