Biopolym. Cell. 2014; 30(6):436-442.
http://dx.doi.org/10.7124/bc.0008BD
Structure and Function of Biopolymers
Influence of brassinosteroids on plant cell alternative respiration pathway and antioxidant systems activity under abiotic stress conditions
1Derevyanchuk M. V., 2Grabelnyh O. I., 3Litvinovskaya R. P., 2Voinikov V. K., 3Sauchuk A. L., 3Khripach V. A., 1Kravets V. S.
  1. Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
    1, Murmans'ka Str., Kyiv, Ukraine, 02094
  2. Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences
    132, Lermontova Str., Irkutsk, Russian Federation, 664033
  3. Institute of Bioorganic Chemistry, NAS of Belarus
    5/2, Kuprevich Str., Minsk, Republic of Belarus, 220141

Abstract

Aim. To investigate the brassinosteroids (BRs) influence on the plant alternative respiration pathway and antioxidant systems to regulate the ROS (reactive oxygen species) production under optimal and abiotic stress conditions. Methods. Respiration measurement experiments were done with the polarographic technique. Original methods were used to evaluate the antioxidant systems activity. Results. Treatment with BRs increased the inten- sity of plant alternative respiration pathway under control and stress conditions. BRs had no effect on alternative respiration of the BR-insensitive bri1–6 plants. Brassinosteroids also increased the activity of a range of antioxidant systems under osmotic stress. Conclusions. BRs are involved in the regulation of alternative respiration pathway and antioxidant systems activity in plant cells under optimal and abiotic stress conditions.
Keywords: brassinosteroids, alternative oxidase, salt stress, osmotic stress, A.thaliana
Full text: (PDF, in English)

References

[1] Khripach V, Zhabinskii V, De Groot A. Twenty years of brassinosteroids: steroidal plant hormones warrant better crops for the XXI century. Ann Bot. 2000; 86(3): 441–7.
[2] Goda H, Sawa S, Asami T, Fujioka S, Shimada Y, Yoshida S. Comprehensive comparison of auxin-regulated and brassinosteroid-regulated genes in Arabidopsis. Plant Physiol. 2004;134(4):1555-73.
[3] Kagale S, Divi UK, Krochko JE, Keller WA, Krishna P. Brassinosteroid confers tolerance in Arabidopsis thaliana and Brassica napus to a range of abiotic stresses. Planta. 2007;225(2):353-64.
[4] Ahammed GJ, Choudhary SP, Chen S, Xia X, Shi K, Zhou Y, Yu J. Role of brassinosteroids in alleviation of phenanthrene-cadmium co-contamination-induced photosynthetic inhibition and oxidative stress in tomato. J Exp Bot. 2013;64(1):199-213.
[5] Dhaubhadel S, Browning KS, Gallie DR, Krishna P. Brassinosteroid functions to protect the translational machinery and heat-shock protein synthesis following thermal stress. Plant J. 2002;29(6):681-91.
[6] Wang ZY. Brassinosteroids modulate plant immunity at multiple levels. Proc Natl Acad Sci U S A. 2012;109(1):7-8.
[7] Kaur R, Ohri P, Bhardwaj R. Effect of 28-homobrassinolide on susceptible and resistant cultivars of tomato after nematode inoculation. Plant Growth Regul. 2013; 71(3): 199–205.
[8] Ali SS, Kumar GB, Khan M, Doohan FM. Brassinosteroid enhances resistance to fusarium diseases of barley. Phytopathology. 2013;103(12):1260-7.
[9] Searle SY, Thomas S, Griffin KL, Horton T, Kornfeld A, Yakir D, Hurry V, Turnbull MH. Leaf respiration and alternative oxidase in field-grown alpine grasses respond to natural changes in temperature and light. New Phytol. 2011;189(4):1027-39.
[10] Van Aken O, Giraud E, Clifton R, Whelan J. Alternative oxidase: a target and regulator of stress responses. Physiol Plant. 2009;137(4):354-61.
[11] Cvetkovska M, Vanlerberghe GC. Alternative oxidase modulates leaf mitochondrial concentrations of superoxide and nitric oxide. New Phytol. 2012;195(1):32-9.
[12] Sieger SM, Kristensen BK, Robson CA, Amirsadeghi S, Eng EW, Abdel-Mesih A, M?ller IM, Vanlerberghe GC. The role of alternative oxidase in modulating carbon use efficiency and growth during macronutrient stress in tobacco cells. J Exp Bot. 2005;56(416):1499-515.
[13] Mhadhbi H, Fotopoulos V, Mylona PV, Jebara M, Aouani ME, Polidoros AN. Alternative oxidase 1 (Aox1) gene expression in roots of Medicago truncatula is a genotype-specific component of salt stress tolerance. J Plant Physiol. 2013;170(1):111-4.
[14] Wang J, Vanlerberghe GC. A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress. Physiol Plant. 2013; 149(4):461–73
[15] Panda SK, Sahoo L, Katsuhara M, Matsumoto H. Overexpression of alternative oxidase gene confers aluminum tolerance by altering the respiratory capacity and the response to oxidative stress in tobacco cells. Mol Biotechnol. 2013;54(2):551-63.
[16] Liu J, Li Z, Wang Y, Xing D. Overexpression of ALTERNATIVE OXIDASE1a alleviates mitochondria-dependent programmed cell death induced by aluminium phytotoxicity in Arabidopsis. J Exp Bot. 2014;65(15):4465-78.
[17] Cvetkovska M, Vanlerberghe GC. Coordination of a mitochondrial superoxide burst during the hypersensitive response to bacterial pathogen in Nicotiana tabacum. Plant Cell Environ. 2012;35(6):1121-36.
[18] Cvetkovska M, Vanlerberghe GC. Alternative oxidase impacts the plant response to biotic stress by influencing the mitochondrial generation of reactive oxygen species. Plant Cell Environ. 2013;36(3):721-32.
[19] Smith CA, Melino VJ, Sweetman C, Soole KL. Manipulation of alternative oxidase can influence salt tolerance in Arabidopsis thaliana. Physiol Plant. 2009;137(4):459-72.
[20] de Azevedo Neto AD, Prisco JT, Eneas-Filho J, de Abreu CEB, Gomes-Filho E. Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and saltsensitive maize genotypes. Environ Exp Bot. 2006; 56(1):87–94.
[21] Monnet F, Bordas F, Deluchat V, Baudu M. Toxicity of copper excess on the lichen Dermatocarpon luridum: antioxidant enzyme activities. Chemosphere. 2006;65(10):1806-13.
[22] Pinto Mdel C, Tejeda A, Duque AL, Mac?as P. Determination of lipoxygenase activity in plant extracts using a modified ferrous oxidation-xylenol orange assay. J Agric Food Chem. 2007;55(15):5956-9.
[23] Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.
[24] Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water-stress studies. Plant Soil. 1973; 39(1):205–7.
[25] Yu C-W, Murphy TM, Sung W-W, Lin C-H. H2O2 treatment induces glutathione accumulation and chilling tolerance in mung bean. Funct Plant Biol. 2002; 29(9):1081-7.
[26] Moller IM, Berczi A, van der Plas LHW, Lambers H. Measurement of the activity and capacity of the alternative pathway in intact plant tissues: Identification of problems and possible solutions. Physiol Plant. 1988; 72(3): 642–9.
[27] Smirnoff N, Cumbes QJ. Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 1989; 28(4):1057–60.
[28] Queval G, Jaillard D, Zechmann B, Noctor G. Increased intracellular H2O2 availability preferentially drives glutathione accumulation in vacuoles and chloroplasts. Plant Cell Environ. 2011;34 (1):21–32.
[29] Zamocky M, Furtm?ller PG, Obinger C. Evolution of catalases from bacteria to humans. Antioxid Redox Signal. 2008;10(9):1527-48.
[30] Jacoby RP, Taylor NL, Millar AH. The role of mitochondrial respiration in salinity tolerance. Trends Plant Sci. 2011;16(11):614-23.
[31] Hare PD, Cress WA. Metabolic implications of stress-induced proline accumulation in plants. Plant Growth Regul. 1997;21 (2):79–102.
[32] Hamilton EW 3rd, Heckathorn SA. Mitochondrial adaptations to NaCl. Complex I is protected by anti-oxidants and small heat shock proteins, whereas complex II is protected by proline and betaine. Plant Physiol. 2001;126(3):1266-74.
[33] Dubreuil-Maurizi C, Vitecek J, Marty L, Branciard L, Frettinger P, Wendehenne D, Meyer AJ, Mauch F, Poinssot B. Glutathione deficiency of the Arabidopsis mutant pad2-1 affects oxidative stress-related events, defense gene expression, and the hypersensitive response. Plant Physiol. 2011;157(4):2000-12.
[34] Cui JX, Zhou YH, Ding JG, Xia XJ, Shi K, Chen SC, Asami T, Chen Z, Yu JQ. Role of nitric oxide in hydrogen peroxide-dependent induction of abiotic stress tolerance by brassinosteroids in cucumber. Plant Cell Environ. 2011;34(2):347-58.
[35] Cao S, Xu Q, Cao Y, Qian K, An K, Zhu Y, et al. Loss-of-function mutations in DET2 gene lead to an enhanced resistance to oxidative stress in Arabidopsis. Physiol Plant. 2005;123(1):57–66.
[36] Zeng H, Tang Q, Hua X. Arabidopsis brassinosteroid mutants det2-1 and bin2-1 display altered salt tolerance. J Plant Growth Regul. 2010; 29(1): 44–52.
[37] Vergara R, Parada F, Rubio S, P?rez FJ. Hypoxia induces H2O2 production and activates antioxidant defence system in grapevine buds through mediation of H2O2 and ethylene. J Exp Bot. 2012;63(11):4123-31.
[38] Cvetkovska M, Alber NA, Vanlerberghe GC. The signaling role of a mitochondrial superoxide burst during stress. Plant Signal Behav. 2013;8(1):e22749.