Biopolym. Cell. 2014; 30(4):279-285.
http://dx.doi.org/10.7124/bc.0008A1
Structure and Function of Biopolymers
Study of dephosphorylated 2'-5'-linked oligoadenylates impact on apo-S100A1 protein conformation by heteronuclear NMR and circular dichroism
1Skorobogatov O. Yu., 1Lozhko D. N., 2, 3Zhukov I. Yu., 1Kozlov O. V., 1Tkachuk Z. Yu.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of Biochemistry and Biophysics, Polish Academy of Sciences
    5a, Pawinskiego, Warsaw, Poland, 02-106
  3. NanoBioMedical Centre, Adam Mickiewicz University
    85, Umultowska Str., 61-614 Poznan, Poland

Abstract

Low molecular weight natural mediators, 2'-5'-linked oligoadenylates, play an important role in interferon-based antiviral mechanism; participate in growth, apoptosis and other important cellular processes. The aim of current study was to find the evidence for the cell interaction with human apo-S100A1 using the methods of circular dichroism (CD) and heteronuclear NMR spectroscopy. Taking into account their concentration within living cells, the 2'-5'A3 oligoadenylates may act as additional biologically active substrates, capable of regulating the S100A1 protein functioning in vivo. The obtained results demonstrated the occurrence of the secondary structure changes in human S100A1 protein upon the interaction with 2'-5'-linked oligoadenylates as well as indicated specific residues involved in this process. Our study points to the 2'-5'-linked oligoadenylates as possible additional elements of the complex system of fine regulation of the Ca2+-signal transduction pathway in human cells.
Keywords: 2'-5'-linked oligoadenylates, S100A1
Full text: (PDF, in English)

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