Biopolym. Cell. 2014; 30(2):118-121.
http://dx.doi.org/10.7124/bc.000889
Genomics, Transcriptomics and Proteomics
Mesenchymal and trophoblast immunophenotype of multipotent stromal cells from human placenta
1, 2Shablii V. A., 1, 2Kuchma M. D., 3Kyryk V. M., 2Svitina H. M., 2Shablii Yu. M., 1Lukash L. L., 2Lobintseva G. S.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of Cell Therapy, Ministry of Health of Ukraine
    3, Kosmonavta Komarova Ave., Kyiv, Ukraine, 03680
  3. State Institute of Genetic and Regenerative Medicine, NAMS Ukraine
    67, Vyshhorodska St., Kyiv, Ukraine, 04114

Abstract

Aim. The investigation of trophoblast markers expression in the mesenchymal stromal cells of placental tissue before and after their isolation and cultivation. Methods. Placental multipotent mesenchymal stromal cells (MMSC) were obtained by culturing the adhesive fraction of cells enzymatically isolated from the placental tissue. The placental derived cells were immunophenotyped by flow cytometry and immunocytochemistry. Results. It was shown that the placental MMSC express the trophoblast markers: human chorionic gonadotropin, pan-cytokeratin, epidermal growth factor receptor HER2. MMSC-like populations of CD90+ CD73+CD45–CD34–CD14–-cells and CD90–CD73+CD45–CD34–CD14–-cells are presented in the placental tissue. Conclusions. The placental MMSC simultaneously express mesenchymal and trophoblast markers.
Keywords: placenta, chorion, multipotent mesenchymal stromal cells, trophoblast, differentiation
Full text: (PDF, in English)

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