Biopolym. Cell. 1987; 3(6):327-329.
Short Communications
The effect of redox potential of the medium on the catalytic activity of NAD-dependent hydrogenase
- A. N. Bakh Institute of Biochemistry, Academy of Sciences of the USSR
Moscow, USSR - M. V. Lomonosov State University
Moscow, USSR
Abstract
The effect of redox potential of the medium on the catalytic properties of soluble hydrogenase from hydrogen-oxidizing bacteria Alcaligenes eutrophus Zl was studied. Two transitions on the catalytic activity of the enzyme vs redox potential profiles are observed: transitions with Em~—300 mV and Em~—400 mV control NAD(H)-dependent activities of enzyme and hydrogen uptake reaction with methyl viologen, respectively. These redox transitions may be accounted for by the model of the intramolecular electron transport chain of hydrogenase. A possible physiological role of the redox regulation of the hydrogenase catalytic activity is discussed. It is assumed that hydrogenase is regulated in vivo by the pool of nicotinamide adenine dinucleotides.
Full text: (PDF, in Russian)
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