Biopolym. Cell. 1987; 3(6):320-322.
Short Communications
A simple method for isolation of haploid protoplasts from forms of rice multishoots
- Institute of Experimental Biology
Ho Chi Minh City, Vietnam
Abstract
One-month old sterile plantlets grown on the 0.3 M sucrose medium were used for protoplast isolation. The young part of leaves were cut onto small pices (1 mm) and left for over night incubation in the 2 % v/v driselase solution containing glucose 0.4 M. Isolated protoplasts were purified by two-phase centrifugation (the upper phase contains solution of salts, the lower one — 0.4 M of sucrose and other nutritional constituents). The purified protoplasts were suspended between two phases.
Full text: (PDF, in Russian)
References
[1]
Chu CC, Wang CC, Sun CS et al. Establishment of an efficient medium for anther culture of rice through comparative experiments on nitrogen sources. Sci Sinica. 1975; N 5:659-668.
[2]
King P, Potrykus I, Thomas E. In vitro genetics of cereals: problem and perspectives. Physiol Veg. 1978; 16(2):381-399.
[3]
Dunstan DI, Short KC, Dhaliwal H, Thomas E. Further studies on plantlet production from cultured tissues ofSorghum bicolor. Protoplasma. 1979; 101(4):355-361.
[4]
Thomas E, King P, Potrykus I. Improvement of crop plants via single cells in vitro an assessment. Z. Pflanzenzucht. 1979; 82(1):1-30.
[5]
Vasil I. K. Regeneration of plants from single cells of cereals and grasses. Genetic engineering in eukaryotes. Eds P. F. Lurquin, A. Kleinhofs. New York : Plenum PubL Co., 1983:233-252.
[6]
Gleba YuYu, Sitnik KM. Cell engineering of plants. Kiev, Naukova Dumka, 1984; 160 p.
[7]
Murashige T, Skoog F. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum. 1962. 15(3):473-497.
