Biopolym. Cell. 1987; 3(6):283-289.
http://dx.doi.org/10.7124/bc.0001FE
Structure and Function of Biopolymers
Interaction between EcoRII restriction/modification enzymes and synthetic DNA fragments. Synthesis of substrates containing a single recognition site
1Kuznetsova S. A., 1Kubareva E. A., 1Oretskaya T. S., 2Dolinnaya N. G., 1Krynetskaya N. F., 1Gromova E. S., 1Shabarova Z. A., 2Cech D.
  1. M. V. Lomonosov State University
    Moscow, USSR
  2. A. Humboldt University
    Berlin, GDR

Abstract

9-16 membered oligodeoxyribonucleotides forming DNA-duplexes with one EcoRII site (native or modified) were synthesized by the block triester method. The modifications involved the replacement of one (or two) cytidine or thymidine moieties in duplexes for m5dC or f5dU, respectively. 30-membered DNA-duplex was obtained by enzymatic ligation of five overlapping oligonucleotides. The substitutions introduced neither result in any significant destabilization nor distort the double helix geometry as is evidenced by the UV- and CD,-spectroscopy methods.
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