Biopolym. Cell. 1987; 3(5):276-279.
Gene-Engineering Biotechnology
Chimeric rpoC'- lacZ' gene of the recombinant pUC19 plasmid reserving the β-galactosidase activity in Escherichia coli cells
1Paton E. B., 1Zhyvoloup A. N.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


A recombinant pUC19/B plasinid was obtained with the pUC19 plasmid and pJC703 cosmid. The inserted pJC703 Bglll-B-hagment codes for rifampicin-resistant E. coli RNA polymerase β-subunit. The rpoC gene fragment is fused to the vector lacZ' gene providing reservation of the β-galactosidase activity in the recombinant cells as a result of chimeric rpoC'-lacZ' gene formation.


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