Biopolym. Cell. 1987; 3(5):234-240.
Structure and Function of Biopolymers
The complexing of ethidium bromide and DNA in moist films as revealed by IR-spectroscopy
- Institute of Radiophysics and Electronics, Academy of Sciences of the Ukrainian SSR
IR-spectroscopy is shown to be a tool in investigating the complexing of ethidium bromide and DNA moist films. The dependence of DNA backbone frequencies and intensities of absorption bands upon water content was analyzed. It is shown that at humidities higher than 56 % the DNA helix formation is accompanied by selective intercalation of ethidium bromide from the narrow groove side. This process alters the DNA structure and leads to a dehydration of nucleic base aminogroups. It is also found that the intercalation prevents from the dipole-dipole interactions between the carbonyl guanine groups.
 Sanenov NAm, Sukhorukov BI, Maleev VIa. Are nitrogen bases of DNA hydrated at low humidity? Biofizika. 1981;26(6):979-84.
 Semenov MA. IR spectroscopic manifestation of DNA hydration. Mol. genetics and biophysics. Kiev, Kiev Univ. press, 1984; 25-33.
 Semenov MA. Experimental investigation of the energy contribution of hydration in stabilizing helical structure of DNA. Tez. of reports. posts and posters. I all-union biophys. Meet. Moscow, 1982; 1: 57.
 Semenov MA, Bol'bukh TV, Maleev VIa. Study of the hydratation of the double-helical poly A-poly U complex by IR-spectroscopy and piezogravimetry methods. Biofizika. 1985;30(4):571-7.
 Fuller W, Waring MJ. A molecular model for the interaction of ethidium bromide with deoxyribonucleic acids. Ber Bunsenges Phys Chem. 1967; 68(8):805-8.
 Dougherty G. A single-beam microspectrophotometer suitable for investigating the linear dichroism of DNA-drug fibers. Anal Biochem. 1981;115(1):52-7.
 Miller KJ, Brodzinsky R, Hall S. Interactions of molecules with nucleic acids. IV. Binding energies and conformations of acridine and phenanthridine compounds in the two principal and in several unconstrained dimer-duplex intercalation sites. Biopolymers. 1980;19(11):2091-122.
 Neidle S, Berman HM. X-ray crystallographic studies of nucleic acids and nucleic acid-drug complexes. Prog Biophys Mol Biol. 1983;41(2):43-66. Review.
 Tsai CC, Jain SC, Sobell HM. X-ray crystallographic visualization of drug-nucleic acid intercalative binding: structure of an ethidium-dinucleoside monophosphate crystalline complex, Ethidium: 5-iodouridylyl (3'-5') adenosine. Proc Natl Acad Sci U S A. 1975;72(2):628-32.
 Jain SC, Tsai CC, Sobell HM. Visualization of drug-nucleic acid interactions at atomic resolution. II. Structure of an ethidium/dinucleoside monophosphate crystalline complex, ethidium:5-iodocytidylyl (3'-5') guanosine. J Mol Biol. 1977;114(3):317-31.
 Sobell HM, Tsai CC, Jain SC, Gilbert SG. Visualization of drug-nucleic acid interactions at atomic resolution. III. Unifying structural concepts in understanding drug-DNA interactions and their broader implications in understanding protein-DNA interactions. J Mol Biol. 1977;114(3):333-65.
 Semenov MA, Bolbukh TV. Carbonyl vibration resonance interactions of helical polynucleotides polv(dA)-poly (dT) and polyG poly C. Stud. biophys. 1984; 102(3):215-20.
 Semenov MA, Bolbukh TV. Resonance oscillations in reacting carbonyl spiral and DNA polynucleotides. IV all-union conf. on spectroscopy of biopolymers: Thes. Kharkiv, 1981:162.
 Semenov MA, Bolbukh TV. IR spectroscopic study of complexation with ethidium bromide to DNA in wet films. Conformational changes in the biopolymer solution; Tez. of reports. All-union conf. Tbilisi, 1985; 151.
 Porumb H. The solution spectroscopy of drugs and the drug-nucleic acid interactions. Prog Biophys Mol Biol. 1978;34(3):175-95.
 Bolbukh TV, Semenov MA. Isotherms of polynucleotide hydration. Biofizika. 1985; 30(3):409-13.
 Lu KC, Prohofsky EW, Van Zandt LL. Vibrational modes of A-DNA, B-DNA, and A-RNA backbones: an application of a green-function refinement procedure. Biopolymers. 1977;16(11):2491-506.
 Bolbukh TV, Starikov EB, Semenov MA. The hydration of double-helical poly(2A) films studied by IR-spectroscopy and piezomicrobalance. Stud biophys. 1985; 108(3):199-207.