Biopolym. Cell. 1987; 3(4):201-207.
Structure and Function of Biopolymers
Effect of selective ligand binding on the molecular DNA melting
1Fridman A. S., 1Lando D. Yu.
  1. Institute of Bioorganic Chemistry, Academy of Sciences of the Byelorussian SSR
    Minsk, USSR

Abstract

Short non-interacting ligands with a selective binding have been studied for their influence on the DNA helix-coil transition. It is shown that a direct binding of such ligands gives the same characteristics of the transition as the influence of dissolved substances on the heat DNA denaturation. The difference in characteristics is observed only at high temperature shifts (more than 30 °C) induced by ligands, where the dependence of the melting point on the GC-content of the complex is nonlinear. It is established that ligands induce the same temperature shifts but different behaviour of the dependence of the melting range width on the concentration.

References

[1] Voskoboinik AD, Monaselidze DR, Mgeladze GN, Chanchalashvili ZI, Lazurkin IuS, Frank-kamenetskiÄ­ IM. Study of DNA melting in the region of the inversion of relative stability of AT and GC pairs. Mol Biol (Mosk). 1975;9(5):783-90.
[2] Akhrem AA, Lando DIu. Influence of ligands characteristic of selective binding to a certain type of base pairs on DNA helix-coil transition I. Model. Theory. Mol Biol (Mosk). 1979;13(5):1098-109.
[3] Akhrem AA, Lando DIu. Influence of ligands selective nature of the interaction at the helix - a tangle of DNA containing satellite fraction. Vestsi AN BSSR. Ser. Khim. 1981; (2):13-9.
[4] Akhrem AA, Lando DIu. Effect of selectively reacting ligands on the helix-coil transition of DNA. III. Calculation of the melting curves of DNA-ligand complexes. Mol Biol (Mosk). 1981;15(5):1083-92.
[5] Lando DIu, Kul'ba AM, Akhrem AA. Effect of selectively reacting ligands on the helix-coil transition of DNA. IV. Heat denaturation of DNA in an acid medium. Mol Biol (Mosk). 1981;15(5):1093-1101.
[6] Akhrem AA, Fridman AS, Lando DYu. Theory of helix-coil transition of the heterogeneous DNA-heteroqeneous ligands complexes. Biopolym Cell. 1985; 1(4):171-179
[7] Lyubchenko YL, Frank-Kamenetskii MD, Vologodskii AV, Lazurkin YS, Gause GG Jr. Fine structure of DNA melting curves. Biopolymers. 1976;15(6):1019-36.
[8] Tong BY, Battersby SJ. Melting fine structure of Ï•X174 DNA and its fragments: A theroetical study. Biopolymers. 1978; 17(12):2933-7.
[9] Frank-Kamenetskii MD, Frank-Kamenetskii AD. Accounting for double-stranded in the theory of the helix - coil transition of DNA. Molekulyarnaya biologiya. 1969; 3(3):375-83.
[10] Fridman AS, Lando Diu. Cooperative contact interaction between the ligands adsorbed on DNA, causes a decrease in the width of the melting range. 5th all-union conf. on spectroscopy of biopolymers: Proc. of reports. Kharkov, 1984; 254.
[11] Belintsev BN, Vologodskii AV, Frank-Kamenetskii MD. Influence of base sequence on the stability of the double helix of DNA. Mol Biol (Mosk). 1976;10(4):629-33.