Biopolym. Cell. 1987; 3(3):154-156.
Short Communications
Changes of adp-ribosylation level in rabbit brain chromatin under the estradiol action
1Panosyan G. A., 1Sarkisyan E. G., 2Gevorkyan E. S., 1Karabashyan L. V.
  1. Institute of Experimental Biology, Academy of Sciences of the Armenian SSR
    Yerevan, USSR
  2. Yerevan State University, Department of Molecular Physics and Biophysics,
    Yerevan, USSR


The action of estradiol on ADP-ribosylation level in the brain chromatin of preadolescent rabbits in various periods after he :mone injection has been studied. The level of ADP-ribosylation is shown to increase during the activation of biosynthetic processes and to decrease with the lowering of the rate of these processes. The results obtained point to the possible regulatory role of ADP-ribosylation process in the hormonal activation of genetic apparatus in eucaryotes, in particular in the brain tissue.


[1] Barausov GD Participation of poly (ADP-ribose) in the genetic processes. Usp sovrem genet. 1985; 13:136-172.
[2] Gaal JC, Pearson CK. Eukaryotic nuclear ADP-ribosylation reactions. Biochem J. 1985;230(1):1-18.
[3] Ueda K, Hayaishi O. ADP-ribosylation. Annu Rev Biochem. 1985;54:73-100.
[4] Panosyan G. A., Gevorkyan E. S., Sarkisyan E. G. Induction cholinesterase by estradiol. Physiology and biochemistry of neurotransmitter processes: Proc. III AllUnion conference. Moscow, 1980:152.
[5] Moudgil VK, Kanungo MS. Induction of acetylcholinesterase by 17- estradiol in the brain of rats of various ages. Biochem Biophys Res Commun. 1973;52(3):725-30.
[6] Graziano SL, Huang RC. Chromatographic separation of chick brain chromatin proteins using a SP-sephadez column. Biochemistry. 1971;10(25):4770-7.
[7] Layne E. Spectrophotometric and turbidimetric methods for measuring proteins. Methods Enzymol. 1957; 3:447-54.
[8] Ito S, Shizuta Y, Hayaishi O. Purification and characterization of poly(ADP-ribose) synthetase from calf thymus. J Biol Chem. 1979;254(9):3647-51.
[9] Das BR, Kanungo MS. ADP-ribosylation induced changes in the conformation of the chromatin of the brain of developing rats. Biochem Int. 1986;12(2):303-11.
[10] Gill DM. Poly (adenosine diphosphate ribose) synthesis in soluble extracts of animal organs. J Biol Chem. 1972;247(18):5964-71.
[11] Katzenellenbogen BS, Bhakoo HS, Ferguson ER, Lan NC, Tatee T, Tsai TS, Katzenellenbogen JA. Estrogen and antiestrogen action in reproductive tissues and tumors. Recent Prog Horm Res. 1979;35:259-300.
[12] Jackowski G, Kun E. The influence of triiodothyronine on polyadenosine-diphosphoribose polymerase and RNA synthesis in cardiocyte nuclei. J Mol Cell Cardiol. 1982;14 Suppl 3:65-70.
[13] Kitamura A, Tanigawa Y, Yamamoto T, Kawamura M, Doi S, Shimoyama M. Glucocorticoid-induced reduction of poly (ADP-ribose) synthetase in nuclei from chick embryo liver. Biochem Biophys Res Commun. 1979;87(3):725-33.
[14] Nikodem VM, Huang DR, Trus BL, Rall JE. The effects of thyroid hormone on in vitro phosphorylation, acetylation, and ADP ribosylation of rat liver nuclear proteins. Horm Metab Res. 1983;15(11):550-4.
[15] Tanuma S, Johnson LD, Johnson GS. ADP-ribosylation of chromosomal proteins and mouse mammary tumor virus gene expression. Glucocorticoids rapidly decrease endogenous ADP-ribosylation of nonhistone high mobility group 14 and 17 proteins. J Biol Chem. 1983;258(24):15371-5.