Biopolym. Cell. 1987; 3(2):72-76.
http://dx.doi.org/10.7124/bc.0001D7
Structure and Function of Biopolymers
Polyelegtrolyte properties of DNA: activation energy of conductivity and conductometric melting curves of concentrated salt-free solutions
1Kuznetsov I. A., 1Apolonnik N. V., 1Shklover I. S.
  1. M. V. Lomonosov State University
    Moscow, USSR

Abstract

Three linear regions on the dependence of specific conductivity of native NaDNA on the temperature in the Arrhenius coordinates are found. These regions of conductometric curve are attributed to the conductivity of native, denatured DNA and to the intermediate structure. The activation energy (Ea) independence on polyion structure is shown. The Ea values for native and denatured NaDNA are found to be equal to 16.4В±1.1 kJ/mole. A share of counterions eliminating with denaturation is approximately evaluated. It is proposed to use conductometry for the obtaining of melting curves of DNA in the concentrated salt-free solutions.
Full text: (PDF, in Russian)

References

[1] Erdey-Gruz T. Transport phenomena in aqueous solutions. Moscow, Mir, 1976; 595 p.
[2] Grassi H, Rix-Montel MA, Kranck H, Vasilescu D. Premelting effects in DNA under saltfree conditions. Biopolymers. 1975; 14(12):2525-35.
[3] Akhrem AA, Andrianov VT, Vlasov AP, Korolev NI, Kuznetsov IA. Thermal denaturation of DNA in concentrated salt-free solutions: comparison of microcalorimetric and spectrophotometric data. Mol Biol (Mosk). 1985;19(3):623-8.
[4] Kuznetsov IA, Apolonnik NV. The equivalent conductivity of aqueous salt-free solutions of DNA: Influence of univalent counterions. Biopolymers. 1981; 20(10):2083-2091.
[5] Spirin AS. Spectrophotometric determination of total nucleic acids. Biokhimiia. 1958;23(5):656-62.
[6] Kuznetsov IA, Filippov SM, Vorontsova OV. Temperature dependence of conductance of isoionic DNA solutions. Determination of dissociation constants of primary phosphoryl groups. Mol Biol (Mosk). 1979;13(3):543-9.
[7] Korolev NI. Influence of counterions on DNA status of concentrated salt-free systems: Avtoref. dis. ... kand. khim. nauk. M., 1985. 17 p.
[8] Mingot F. Quantitative test of record's theory for proton-induced lowering of DNA melting temperature. Biopolymers. 1981;20(10):2121-36.