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Genome and Its Regulation
Liposome-mediated delivery of DNA to Saccharomyces cerevisiae protoplasts
- Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
To determine the peculiarities of plasmid DNA delivery by liposomes to yeast protoplasts (Saccharomyces cerevisiae) plasmid RB4 was encapsulated into large monolamellar liposomes from lecithin and cholesterin (mol, ratio 5:1). Fusion of liposomes with protoplasts was carried out. The uptake of plasmid DNA incorporated into liposomes by competent yeast cells was shown to exceed 8–12 fold than of free DNA. Liposomes without incorporated DNA were found to promote transformation of S. cerevisiae protoplasts 5–8 fold, if DNA concentration in a sample was 20–40 (μg/ml. The effect was absent at higher DNA concentrations in a sample (150–200 μg/ml).
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