Biopolym. Cell. 1987; 3(1):35-40.
http://dx.doi.org/10.7124/bc.0001D1
Genome and Its Regulation
Liposome-mediated delivery of DNA to Saccharomyces cerevisiae protoplasts
1Kostetsky I. E., 1Kordyum V. A.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

To determine the peculiarities of plasmid DNA delivery by liposomes to yeast protoplasts (Saccharomyces cerevisiae) plasmid RB4 was encapsulated into large monolamellar liposomes from lecithin and cholesterin (mol, ratio 5:1). Fusion of liposomes with protoplasts was carried out. The uptake of plasmid DNA incorporated into liposomes by competent yeast cells was shown to exceed 8–12 fold than of free DNA. Liposomes without incorporated DNA were found to promote transformation of S. cerevisiae protoplasts 5–8 fold, if DNA concentration in a sample was 20–40 (μg/ml. The effect was absent at higher DNA concentrations in a sample (150–200 μg/ml).
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References

[1] Papahajopolos O., Wilson T., Taber R. Liposomes as macromolecular for the introduction of RNA and DNA into cells. Transfer cell const, eukaryotic cells: Lect. NATO adv. study Inst. (Sintra-Estoril, 1979). New York; London, 1980:155-172.
[2] Lurquin PF, Shehy RE. Effects of conditions of incubation on the binding of DNA-loaded liposomes to plant protoplasts. Plant Sci. Lett - 1982; 28(1):49-61.
[3] Holubova I, Jandova Z, Tichy P, Hubacek J, Ludvik J. Transfer of liposome-encapsulated plasmid DNA to Bacillus subtilis protoplasts and calcium-treated Escherichia coli cells. Folia Microbiol (Praha). 1985;30(2):97-100.
[4] Glumova EF, Prozorov AA. Transformation of competent Bacillus subtilis cells by chromosomal and plasmid DNA incorporated in liposomes]. Genetika. 1983;19(12):1958-64.
[5] Gregoriadis G, Allison A. Liposomes in biological systems. Moscow, Medicine, 1983; 384 p.
[6] Botstein D, Falco SC, Stewart SE, Brennan M, Scherer S, Stinchcomb DT, Struhl K, Davis RW. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene. 1979;8(1):17-24.
[7] Cates M. Tech lipidology. Moscow, Mir, 1975; 258 p.
[8] Deamer D, Bangham AD. Large volume liposomes by an ether vaporization method. Biochim Biophys Acta. 1976;443(3):629-34.
[9] Beliaev ND, Budker VG, Maksimova TG, Naumova LP, Sokolov AV. Transfection of bacterial cells by DNA of phage M13 entrapped in phospholipid vesicles. Mol Biol (Mosk). 1982;16(3):612-8.
[10] Gorlov YuI, Kirillova VS. Methods for producing yeast protoplasts. Molekulyarnaya biologiya. 1978; Is. 20:85-94.
[11] Ahn JS, Pack MY. Use of liposomes in transforming yeast cells. Biotechnol. Lett. 1985. 7(8):553-6.
[12] Rodicio MR, Chater KF. Small DNA-free liposomes stimulate transfection of streptomyces protoplasts. J Bacteriol. 1982;151(3):1078-85.