Biopolym. Cell. 2013; 29(6):487-492.
Genomics, Transcriptomics and Proteomics
Genetic diversity of the gene pool of bread winter wheat alleles of microsatellite locus Xgwm182-5D associated with frost resistance
1Galaeva M. V., 1Fayt V. I., 1Sivolap Yu. M.
  1. Plant breeding and Genetics Institute, National Centre of Seed and Cultivar Investigation
    Ovidiopol'skaya dor., 3, Odessa, Ukraine, 65036

Abstract

Aim. Identification of winter wheat varieties from different regions at Xgwm182-5D locus. Methods. Polymerase chain reaction (PCR), gel-electrophoresis. Results. 180 genotypes of winter wheat varieties from Ukraine and Russia selection centers are identified by the locus Xgwm182-5D. There were detected five alleles of this locus, namely 162, 165, 167, 169 and 174 bp. Allele 165 bp was noted to be more common in the sets of investigated varieties in all regions. Allele 162 bp was identified only in the sets of varieties of Southern Ukraine and North Caucasus (20 % and 4 %, respectively). Allele 169 bp was detected only in varieties of Western Siberia and Volga region. Conclusions. The differences in frequencies of locus Xgwm182-5D alleles may be due to adaptive and breeding value of these alleles for the conditions of certain regions.
Keywords: winter wheat, frost resistance, microsatellite loci

References

[1] Lytvynenko N., Lyfenko S., Druzyak V., Druzyak V. Influence of crop terms and sublethal winter temperatures upon a survival and productivity of winter wheat News of Agrarian Sciences 2004 N 5 P. 27–31.
[2] Lyfenko S. Ph. Some patterns of inheritance of frostresistance in winter wheat hybrids The ways of creating of initial material for breeding of cereal crops Odessa: PBGI, 1976 Vol. 14 P. 71–86.
[3] Litvinenko M. A. The wheat breeding and seed production department in the 100-year history of the institute Collected scientific articles in Ukrainian of PBGI-NCSCI 2012 20 (60) P. 11–27.
[4] Peleman J. D., van der Voort J. R. The challenges in marker assisted breeding Eucarpia Leafy Vegetables: Proc. EUCARPIA Meet. on Leafy Vegetables Genetics and Breeding / Eds Th. J. L. van Hintum, A. Lebeda, D. Pink, J. W. Schut Noordwijkerhout, 2003:125–130.
[5] Sutka J., Galiba G., Veisz O., Snape J. Genetic analysis of frost resistance and its contribution to development of frost resistant cereal varieties: a review Plant Breeding and Seed Science 1997 41, N 2:39–50.
[6] Galiba G., Quarrie S. A., Sutka J., Morgounov A., Snape J. W. RFLP mapping of the vernalization (Vrn1) and frost resistance (Fr1) genes on chromosome 5A of wheat Theor. Appl. Genet 1995 90:1174–1179.
[7] Snape J. W., Semikhodskii A., Fish L., Sarma R. N., Quarrie S. A., Galiba G., Sutka J. Mapping of frost tolerance loci in wheat and comparative mapping with other cereals Acta Agron. Hung 1997 45:268–270. wheat Theor. Appl. Genet 1995 90:1174–1179.
[8] Toth B., Galiba G., Feher E., Sutka J., Snape J. W. Mapping genes affecting flowering time and frost resistance on chromosome 5B of wheat Theor. Appl. Genet 2003 107:509–514.
[9] Vagujfalri A., Galiba G., Cattivelli L., Dubcovsky J. The coldregulated transcriptional activator Cbf3 is linked to the frosttolerance locus Fr-A2 on wheat chromosome 5A Mol. Genet. Genomics 2003 269:60–67.
[10] Galaeva M. V., Fayt V. I., Chebotar S. V., Galaev A. V., Sivolap Yu. M. Association of microsatellite loci alleles of the group-5 chromosomes with the frost resistance of winter wheat Cytology and Genetics 2013 47, N 5:261–267.
[11] Application of PCR analysis in genetic and breeding research: Scientific-method. Manual Kyiv: Agrar. Nauka, 1998 156 p.
[12] Rokitsky P. F. Biological statistics Moscow: Kolos, 1973 327 p.