Biopolym. Cell. 2013; 29(5):375-381.
http://dx.doi.org/10.7124/bc.00082C
Structure and Function of Biopolymers
Early detection and group-specific identification of Mycobacterium tuberculosis strains by method of single-nucleotide polymorphism analysis with hairpin primers
1Cherednyk Yu. O., 2Anopryenko O. V., 3Gorovenko N. G., 1Feschenko Yu. I.
  1. National Institute of Phthisiology and Pulmonology named after F. G. Yanovskiy, NAMS
    10, M. Amosova Str., Kyiv, Ukraine, 03680
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  3. P. L. Shupik National medical academy of post-graduate education
    9, Dorohozhytska Str., Kyiv, Ukraine, 04112

Abstract

Aim. Based on the method of single-nucleotide polymorphism (SNP) determination with hairpin primers to perform a differential identification in clinical material of M. tuberculosis (Mtb) strains, which belong to the 1st or 2/3 principal genotypic groups (PGG), with the aim of shortening the terms of tuberculosis diagnosing and early detection of most clinically and epidemiologically-significant strains. Methods. PCR with the SNP-specific hairpin primers to group-specific SNP katG463, and statistical analysis of clinical/epidemiological categories of the patients were used for study of sputum clinical samples from patients with pulmonary tuberculosis living in Kyiv. Results. PCR system of differential group-specific detection of Mtb in clinical samples using hairpin primers to SNP katG463 effectively detected Mtb in 47.8 % of samples of which 57.6 % were strains of the PGG-1 and 42.4 % – PGG-2/3. The association between belonging to the PGG-1 and resistance to iso- niazid (OR [95 % CI], 5.417 [1.196–24.522] P = 0.0283) and to any of the first-line drugs (rifampicin/isoniazid) (OR [95 % CI], 7.00 [1.493– 32.82] P = 0.014) was revealed. Conclusions. SNP-analysis with hairpin SNP-specific primers to locus katG463 of Mtb strains group membership in clinical material allows effective detection of epidemiologically-important PGG-1 strains.
Keywords: SNP, Mycobacterium tuberculosis, katG, PCR-diagnostics, tuberculosis
Full text: (PDF, in Ukrainian)

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