Biopolym. Cell. 2013; 29(2):163-166.
http://dx.doi.org/10.7124/bc.000815
Short Communications
Increasing of antioxidant and superoxide dismutase activity in chicory transgenic plants
1Kvasko O. Yu., 1Matvieieva N. A.
  1. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

Aim. Determination of the antioxidant activity (AOA) and superoxide dismutase (SOD) activity in transgenic chicory plants carrying the human interferon α2b target and nptII or bar selective genes. Methods. AOA was measured by a method based on the determination of kinetics of the reduced 2,6-dichlorophenolindophenol oxidation. SOD activity was assayed using the system consisting of ethionine, riboflavin, and nitroblue tetrazolium. Results. Antioxidant activity of transformed plants extracts was more than 1,91–2,59 and 2,04–2,43 times over the activity of control non-transgenic plants (at nptII and bar gene presence respectively). SOD activity was higher in transgenic plants than in the control, and was 2,03 ± 0,46–3,33 ± 0,54 U/g weight (nptII gene) and 2,25 ± 0,46–2,68 ± 0,08 U/g weight (bar gene). Conclusions. Transgenic C. intybus plants have higher antioxidant and superoxide dismutase activity compared to non-transgenic plants. The increasing of AOA and SOD activity is a response of plants to transformation stress factor and integration of foreign genes in plant genome.
Keywords: genetic transformation, Cichorium intybus, antioxidant activity, superoxide dismutase activity
Full text: (PDF, in English) (PDF, in Ukrainian)

References

[1] Haslberger A. G. Codex guidelines for GM foods include the analysis of unintended effects Nat. Biotech 2003 21, N 7. – P. 739-741.
[2] Prakash C. S. The genetically modified crop debate in the context of agricultural evolution Plant Physiol 2001 126, N 1 P. 8–15.
[3] Shewmaker C. K., Sheehy J. A., Daley M., Colburn S., Ke D. Y. Seed-specific overexpression of phytoene synthase: increase in carotenoids and other metabolic effects Plant J 1999 20, N 4 P. 401–412X.
[4] Enikeev A. G., Kopytina T. V., Semenova L. A., Natyaganova A. V., Gamanetz L. V., Volkova O. D. Agrobacterial transformation as complex biotical stressing factor J. Stress Physiol. Biochem 2008 4, N 1:11–19.
[5] Mittler R. Oxidative stress, antioxidants and stress tolerance Trends Plant Sci 2002 7, N 9 P. 405–410.
[6] Bowler C., Montagu M. V., Inze D. Superoxide dismutases and stress tolerance Annu. Rev. Plant Physiol. Plant Mol. Biol 1992 43 P. 83–116.
[7] Matvieieva N. A., Shachovsky A. M., Gerasymenko I. M., Kvasko O. Yu., Kuchuk N. V. Agrobacterium-mediated transformation of Cichorium intybus L. with interferon-a2b gene Biopolym. Cell 2009 25, N 2 P. 120–125.
[8] Kvasko O. Y., Matvieieva N. A., Shahovsky A. M., Kuchuk N. V. Obtaining of transgenic endive Cichorium endivia L. and chicory C. intybus L. plants. The Bull. Vavilov Soc. Geneticists and Breeders of Ukraine 2012 10, N 1:28–32.
[9] Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant. 1962;15(3):473–97.
[10] Semenov V. L., Yarosh A. M. A method of determining the antioxidative activity of biological matter Ukr. Biokhim. Zh 1985 57, N 3:50–52.
[11] Giannopolities C. N., Ries S. K. Superoxid dismutase. I. Occurrence in higher plants Plant Physiol 1977 59, N 2:309–314.
[12] Mittova V., Tal M., Volokita M., Guy M. Up-regulation of the leaf mitochondrial and peroxisomal antioxidative systems in response to salt-induced oxidative stress in the wild salt-tolerant tomato species Lycopersicon pennellii Plant Cell Environ 2003 26, N 6:845–856.
[13] Alonso R., Elvira S., Castillo F. J., Gimeno B. S. Interactive effects of ozone and drought stress on pigments and activities of antioxidative enzymes in Pinus halepensis Plant Cell Environ 2001 24, N 9 P. 905–916.