Biopolym. Cell. 2013; 29(2):143-149.
Viruses and Cell
Phylogenetic analysis of influenza A(H1N1)pdm
viruses isolated in Ukraine during the 2009–2010
pandemic season
1, 2Leibenko L. V., 1Polischuk V. P., 2Mironenko A. P.
- Educational and Scientific Center "Institute of Biology",
Taras Shevchenko National University of Kyiv
64/13, Volodymyrska Str., Kyiv, Ukraine, 01601 - Gromashevsky L.V. Institute of Epidemiology and Infection Diseases, AMS of Ukraine
5, Amosova Str., Kyiv, Ukraine, 03038
Abstract
Aim. To perform the phylogenetic analysis of segments, encoding hemagglutinin and neuraminidase of A(H1N1) pdm influenza viruses, isolated in Ukraine. Methods. In this study the real-time polymerase chain reaction (RT-PCR), sequencing and phylogenetic analysis methods were used. Results. Key mutations in amino acid sequences of proteins of Ukrainian pandemic influenza isolates were analyzed. High genetic similarity of Ukrainian and foreign pandemic isolates (99 %) was observed. Conclusions. The stability of Ukrainian isolates genes during 2009–2010 pandemic season was shown.
Keywords: phylogenetic analysis, nucleotide sequence, A(H1N1)pdm influenza viruses
Full text: (PDF, in English)
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