Biopolym. Cell. 2012; 28(6):429-433.
Structure and Function of Biopolymers
Alternatively spliced short and long isoforms of adaptor protein intersectin 1 form complexes in mammalian cells
1, 2Gubar O. S., 1Kropyvko S. V., 1Tsyba L. O., 2Gasman S., 1Rynditch A. V.
- State Key Laboratory of Molecular and Cellular Biology
Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 - Institut des Neurosciences Cellulaires et Integratives (INCI) UPR 3212, CNRS -Universite de Strasbourg, Centre de Neurochimie
5, rue Blaise Pascal, Strasbourg, France, 67084
Abstract
Intersectin 1 (ITSN1) is an adaptor protein involved in membrane trafficking and cell signaling. Long and short isoforms of ITSN1 (ITSN1-L and ITSN1-S) are produced by alternative splicing. The aim of our study was to investigate whether ITSN1-L and ITSN1-S could interact in mammalian cells. Methods. During this study we employed immunoprecipitation and confocal microscopy. Results. We have shown that endogenous ITSN1-S co-precipitates with overexpressed ITSN1-L in PC12, 293 and 293T cells. Long and short isoforms of ITSN1 also co-localize in 293T cells. Conclusions. ITSN1-L and ITSN1-S form complexes in mammalian cells.
Keywords: ITSN1, alternatively spliced isoforms, adaptor/scaffold proteins
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