Biopolym. Cell. 2012; 28(6):429-433.
http://dx.doi.org/10.7124/bc.000132
Structure and Function of Biopolymers
Alternatively spliced short and long isoforms of adaptor protein intersectin 1 form complexes in mammalian cells
1, 2Gubar O. S., 1Kropyvko S. V., 1Tsyba L. O., 2Gasman S., 1Rynditch A. V.
  1. State Key Laboratory of Molecular and Cellular Biology
    Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institut des Neurosciences Cellulaires et Integratives (INCI) UPR 3212, CNRS -Universite de Strasbourg, Centre de Neurochimie
    5, rue Blaise Pascal, Strasbourg, France, 67084

Abstract

Intersectin 1 (ITSN1) is an adaptor protein involved in membrane trafficking and cell signaling. Long and short isoforms of ITSN1 (ITSN1-L and ITSN1-S) are produced by alternative splicing. The aim of our study was to investigate whether ITSN1-L and ITSN1-S could interact in mammalian cells. Methods. During this study we employed immunoprecipitation and confocal microscopy. Results. We have shown that endogenous ITSN1-S co-precipitates with overexpressed ITSN1-L in PC12, 293 and 293T cells. Long and short isoforms of ITSN1 also co-localize in 293T cells. Conclusions. ITSN1-L and ITSN1-S form complexes in mammalian cells.
Keywords: ITSN1, alternatively spliced isoforms, adaptor/scaffold proteins
Full text: (PDF, in English)

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