Biopolym. Cell. 2012; 28(5):357-362.
http://dx.doi.org/10.7124/bc.000070
Structure and Function of Biopolymers
GTPase-activating protein oligophrenin 1 is a new partner of multifunctional adapter protein intersectin 1
1, 2Gubar O. S., 2Houy S., 3Billuart P., 1Kropyvko S. V., 1Tsyba L. O., 2Gasman S., 1Rynditch A. V.
  1. State Key Laboratory of Molecular and Cellular Biology
    Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institut des Neurosciences Cellulaires et Integratives (INCI) UPR 3212, CNRS -Universite de Strasbourg, Centre de Neurochimie
    5, rue Blaise Pascal, Strasbourg, France, 67084
  3. CNRS UMR 8104, Institut Cochin
    24, rue du Faubourg Saint-Jacques, Paris, France, 75014

Abstract

Intersectin 1 (ITSN1) is a multifunctional adaptor protein which is involved in endocytosis, exocytosis and cellular signaling and it is also associated with such pathologies as Down syndrome and Alzheimer’s disease. The aim of this study was to identify new ITSN1 protein partners which are implicated in membrane trafficking. Methods. In silico analysis by Scansite online resource had identified a GTPase activating protein oligophrenin 1 (OPHN1) as a potential partner of ITSN1 SH3A domain. GST pull-down and immunoprecipitation were used to prove complex formation between ITSN1 and OPHN1. Subcellular protein localization was determined by immunofluorescence and confocal microscopy. Results. We have shown that brain-specific and ubiquitously expressed SH3A domain isoforms of ITSN1 interact with OPHN1. ITSN1 and OPHN1 form complexes in both resting and stimulated to exocytosis PC12 cell line. Conclusions. GTPase activating protein OPHN1 and adaptor protein ITSN1 interact in PC12 cell line independently of exocytosis stimulation.
Keywords: intersectin 1, oligophrenin 1, PC12, exocytosis
Full text: (PDF, in Ukrainian)

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