Biopolym. Cell. 2012; 28(5):352-356.
http://dx.doi.org/10.7124/bc.000077
Structure and Function of Biopolymers
Plasminogen/plasmin influence on platelet aggregation
1Roka-Moya Y. M., 1Zhernossekov D. D., 1Grinenko T. V.
  1. Palladin Institute of Biochemistry, NAS of Ukraine
    9, Leontovycha Str., Kyiv, Ukraine, 01601

Abstract

Aim. Determination of the influence of Glu- and Lys-plasminogen/ plasmin on the platelet aggregation induced by different agonists. Methods. Platelet aggregometry. Spectrophotometry. Results. We have shown the inhibitory effect of Lys-plasminogen on human platelet aggregation. The pro-enzyme action is related to the second wave of aggregation. The inhibitory effect of Lys-plasminogen possesses dosedependent manner in case of thrombin-induced aggregation. The inhibitory effect of pro-enzyme with ristocetin as an inductor has not been observed. Glu-plasminogen and plasmin do not influence on platelet aggregation under studied conditions. A serine protease inhibitor aprotinin does not change the inhibitory effect of Lys-plasminogen. Conclusions. The results proved the inhibitory effect of Lys-plasminogen on thrombin- and collagen-induced platelet aggregation. The absence of inhibitory effect of Lys-plasminogen in the case of ristocetin-induced aggregation leads us to conclusion, that GP Ib/IX is not involved into the inhibited aggregation pathway. The unchanged inhibitory effect of Lys-plasminogen in the presence of aprotinin excludes plasmin participation in the inhibitory phenomenon.
Keywords: platelet aggregation, plasminogen, plasmin
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