Biopolym. Cell. 2012; 28(3):207-211.
http://dx.doi.org/10.7124/bc.00004F
Minireviews
Photoreactive DNA as a tool for studying topography of nucleotide excision repair complex
1Rechkunova N. I., 1Krasikova Y. S., 1Maltseva E. A., 1Lavrik O. I.
  1. Novosibirsk Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences
    8, Akademika Lavrentieva Ave., Novosibirsk, Russian Federation, 630090

Abstract

Nucleotide excision repair(NER) is one of the major DNA repair pathwaysin eukaryotic cells preventing genetic abnormalities caused by DNA damage. NER removes a wide set of structurally diverse lesions such as pyrimidine dimers arising upon UV irradiation and bulky chemical adducts arising upon exposure to environmental carcinogens or chemotherapeutic drugs. In view of the extraordinarily broad substrate specificity of NER, it is of interest to understand how a certain set of proteins recognizes various DNA lesions in the context of a large excess of intact DNA. Thisreview focuses on contribution of photoaffinity labeling technique in the study of DNA damage recognition and following stages resulting in preincision complex assembly, the key and still most unclear steps of NER.
Keywords: nucleotide excision repair, damage recognition, preincision complex, photoreactive DNA
Full text: (PDF, in English)

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